摘要
目的获取小动物体内近红外荧光深针的荧光图像,找出体内探测深度与荧光深针浓度两者之间的关系。方法采用低温制冷高灵敏度CCD作为系统的深测器,通过探测器采集出荧光探针在小动物体内,以及液态仿体内不同深度的荧光信号。结果得到了不同浓度的荧光探针在不同探测深度的荧光强度信息。结论找到了小鼠体内不同位置及深度的荧光强度关系。近似地找出了荧光探针浓度与探测强度的线性关系,可作为日后实验系统的参考。
Objective To obtain the near-infrared fluorescence image in vivo and find the relationship between the detecting depth and fluorescence probe concentration. Methods Signals of fluorescence probe in various depths of vivo and tissue phantom with cooled CCD camera were acquired. Results The fluorescence intensity informations with different fluorescence probe concentrations and depths in vivo and liquid phantom were obtained. Conclusion Relationship between fluorescence intensity,location and depth of detecting probe in vivo is found. The linear relation of fluorescence probe concentration and detecting intensity is simulated,which will be used as a reference for the experiment system.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2009年第4期277-280,共4页
Space Medicine & Medical Engineering
基金
863项目基金资助(2006AA020803)
973基金资助项目(2006CB705700)
关键词
荧光分子成像
光学成像
荧光强度
fluorescence molecular imaging
optical imaging
fluorescence intensity
