摘要
目的:基于CCD的荧光分子断层成像(FMT)近年来取得了快速发展,非接触式荧光分子断层成像技术(FMT)需要获取成像对象的三维表面轮廓,用于荧光团浓度重建的前向模型。方法:提出一种在白光条件下基于反投影法的三维轮廓提取方法,在获取360°的CCD照片后,滤波反投影重建断层,经过相应的图像平滑处理,得到轮廓边界,校准后重建出表面轮廓。结果:仿体实验和小动物实验结果表明,该方法具有毫米量级的重建精度。结论:该方法能够很好地重建躯干部分表面三维轮廓,较其他表面轮廓提取方法简单、实用,而且能够顺利地结合到荧光成像的工作流程中去,不需要单独成像,保证了整个成像过程的连续性。
Objective： The CCD-based non-contact fluorescencemolecular tomography has got a rapid progress in recent years, This imaging modality needs to acquire 3D-surface of the animal imaged, which is used as the forward model in the fluorescence concentration reconstruction. Methods： We proposed a new 3D-surface reconstruction method based on the back-projection method under the white light condition. After taking the surface pictures by CCD, the slice images were reconstructed using filter back- projection method, then the edge curve of each slice was extracted, and based on the data of the edges and calibration operation, we reconstructed the 3D-surface of the animal imaged. Results： The results of both phantom simulation and animal experiment showed that the method properly reconstructed the 3D-surface of trunk of small animals. Conclusions： Compared with other methods, this method is simple and fast. Furthermore, the proposed method enables the surface imaging to be inserted into the working process of the whole FMT system without interruption.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2008年第3期360-365,共6页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30670577
60571013
60331010)
国家重点基础研究规划项目(973)(2006CB705700)
清华-裕元基金
