摘要
鉴于与恢复基因紧密连锁的分子标记在分子辅助选择育种中的应用前景,采用SSR标记法定位大豆CMS恢复系WR016的恢复基因。根据(W931A×WR016)F1、F2的植株育性,分析表明大豆M型不育系统为单基因配子体不育。由于大豆M-CMS恢复系WR016的恢复基因定位在A1连锁群上,利用A1连锁群上的大豆SSR引物对不育系W931A和恢复系WR016构建的F2分离群体进行分析,获得了与恢复基因连锁的三个标记Satt684、Satt276和Satt545,遗传距离分别为29.5cM、10.7cM和14.1cM。虽然10.7cM还是一个较远的距离,但为进一步精确定位恢复基因,并最终克隆恢复基因打下了基础。
In view of the application prospects of the molecular marker - assisted selection in breeding by the molecular markers closely linked with restorer gene, this experiment aims to locate restorer gene of the M - CMS WR016 with SSR markers. Fenilities of F1 ,F2generations of W931A（ sterile line） × WROl6（restorer line） showed that M - CMS belonged to single - gene gametophyte sterility. This experiment applies SSR molecular markers to locate the restorer gene of the restorer line WR016. Three SSR markers in A1 linkage group,Satt684,Satt276 and Satt545 ,genetically linked to the restorer gene, were selected by screening of SSR markers with F2 segregation population. The genetic distance between the markers and the restorer gene were 29.5,10.7 and 14.1 cM,respectively. Although 10.7 cM is still a large distance,it laid the foundation for further location of restorer gene.
出处
《大豆科学》
CAS
CSCD
北大核心
2009年第4期578-582,共5页
Soybean Science
基金
国家高技术研究发展计划(863计划)资助项目(2006AA100104)
国家科技支撑计划资助项目(2006BAD01A04)
