摘要
选用装有永久性瘤胃瘘管的３只本地空怀母山羊进行自身对照试验。试验期从瘤胃瘘管灌注半胱胺（１００ｍｇ·ｋｇ－１ＢＷ），分别于灌注前１ｄ（－１ｄ）和灌注后第１，３，５，７ｄ动态采集瘤胃液，测定瘤胃液ｐＨ，氨氮（ＮＨ３－Ｎ），总脱氢酶活性（ＴＤＨＡ），原虫蛋白（Ｐ－Ｐ）和细菌蛋白（Ｂ－Ｐ）的含量。结果表明，ｐＨ于１ｄ明显升高，至７ｄ基本恢复正常；ＴＤＨＡ于３ｄ明显上升，１，２，３号羊分别由－１ｄ的（１０００±０３００）ｎｍｏｌ·ｓ－１·ｍＬ－１，（０６５０±０２３３）ｎｍｏｌ·ｓ－１·ｍＬ－１，（０６６７±０１１７）ｎｍｏｌ·ｓ－１·ｍＬ－１上升到３ｄ的（１６３０±０８８４）ｎｍｏｌ·ｓ－１·ｍＬ－１（Ｐ＜００５），（１１２０±０１８３）ｎｍｏｌ·ｓ－１·ｍＬ－１（Ｐ＜００５）和（１１８０±０２１７）ｎｍｏｌ·ｓ－１·ｍｌ－１（Ｐ＜００１），７ｄ基本恢复正常；ＮＨ３－Ｎ浓度于１ｄ下降，１，２号羊分别由－１ｄ的０．２７８，０１７５ｇ·Ｌ－１下降至１ｄ的０２０２ｇ·Ｌ－１和０１４０ｇ·Ｌ－１，３号羊则由－１ｄ的０３１１ｇ·Ｌ－１降至３ｄ的０２４３ｇ·Ｌ－１；Ｐ－Ｐ含量于１ｄ明显?
The effects of cysteamine infusion into the rumen of goats were studied on the ruminal digestion and metabolism.Three local nonpregnant female goats fixed with permanent ruminal fistulas were used in a self control design.Cysteamine was infused into rumen on d 0 Ruminal fluid was obstained in dymanic state on d -1,1,3,5,7.Compared with the control, the pH value in ruminal fluid apparently increased on d 1;then became normal on d 7;The TDHA in ruminal fluid significantly increased on d 3.Three goats increased from (1000±0300)nmol·s-1·mL-1,(0650±0230)nmol·s-1·mL-1 and (0667±0117)nmol·s-1·mL-1 on d -1 to (1630±0884)nmol·s-1·mL-1(P<005),(1170±0183)nmol·s-1·mL-1(P<005) and (1180±0217)nmol·s-1·mL-1(P<001) on d 3, respectively;The NH3N concentration decreased after intraruminal infusion on d 1, two goats decreased from 0.278 g·L-1, 0.175g·L-1 on d -1 to 0.202 g·L-1,0140 g·L-1 on d 1.Another goat decreased from 0.311 g·L-1 on d -1 to 0.243 g·L-1on d 3;The content of P-P significantly increased on d 1,two goats increased from (0.332±009) g·L-1 and (0.275±0.030)g·L-1 on d -1 to (0.474±0.088)g·L-1(P<001) and (0.372±0.037)g·L-1(P<0.05) on d 1,respectively;another goat not apparently change;The content of P-P decreased significantly on d 7;The content of B-P decreased with the increasing of the content of P-P,and then increased with the decreasing of the contentt of P-P.The results indicated that cysteamine infusion into rumen might elevate the ruminal fermentation and the efficiency of MCP synthesis in rumen.
出处
《河南农业大学学报》
CAS
CSCD
1998年第2期118-121,132,共5页
Journal of Henan Agricultural University
基金
国家自然科学基金
河南省自然科学基金
关键词
半胱胺
瘤胃
消化
代谢
山羊
cysteamine
rumen
digestion
metabolism
