摘要
通过核酸序列比对,在蛇苔cDNA文库中获得细胞色素C(Cyt C)基因序列,并对其编码的蛋白质产物从同源性、氨基酸组成、理化性质、亚细胞位点、结构和功能等进行生物信息学分析和预测。结果表明,该cDNA序列具有完整的开放阅读框(ORF,104～442bp),推测编码蛋白为112个氨基酸,与向日葵、荞麦、玉米等Cyt C存在着较高的保守性(相似性分别为84%、85%和84%),同属于细胞色素C超家族;蛇苔Cyt C蛋白的分子量为11998.7Da,不含信号肽,成熟的Cyt C起始于Ala2,其活性位点为Cys23、Cys26、His27和Met89;对蛋白质的翻译后修饰预测表明Ala2存在乙酰化修饰,Lys81和Lys95分别进行三甲基赖氨酸修饰。这些特点与其他植物Cyt C保持一致,表明该基因为蛇苔cyt c基因,在进化上相对保守。
Through nucleotide blast the cyt c gene was gained from the cDNA library of Conocephalum conicum, and its produce was analyzed in the following aspects：the homology, composition of amino acids, physico-chemical characters, subcellular location, tertiary structure, function and so on by bioinformational tools. The result indicates the sequence has a complete open read frame （104 -442 bp）, and it encodes 112 amino acids which has a high homology with Helianthus annuus, Fagopyrum esculentum, Zea mays and other plants Cyt Cs. They all belong to Cytochrome C superfamily. With molecular weight 11998.7 Da and no signal peptide, the sequence of Conocephalum conicum Cyt C starts Ala2 and takes Cys23 ,Cys26,His27 and Met89 as its active sites. What is more Ala2 is acetylized and the sites Lys81/ Lys95 are modified to be trimethyllysine. All these characteristics keep in line with other plant Cyt Cs, which illuminates the sequence we got is cyt c gene which is conserved in the course of evolution.
出处
《武汉植物学研究》
CAS
CSCD
北大核心
2009年第4期432-436,共5页
Journal of Wuhan Botanical Research
关键词
细胞色素C
蛇苔
序列
功能预测
Cytochrome C （ Cyt C）
Cortocephalum conicum （ L. ） Dum.
Sequence
Function predication
