摘要
目的①研究黄芪甲苷对软骨细胞增殖的促进作用,为软骨组织工程短期内大量种子细胞的获得提供一条新途径;②研究黄芪甲苷对软骨细胞合成细胞内蛋白质和蛋白多糖能力的促进作用,了解黄芪对软骨细胞生理功能发挥的影响。方法经处理后的新西兰大白兔关节细胞分别接种于无药组及浓度分别为5、10、20、40、80、160μg/m l黄芪甲苷组,计数法比较第1～8天细胞增殖情况差异;四甲基偶氮唑盐（MTT）法检测各组软骨细胞活性差异;考马斯亮蓝染色法检测各组一定量细胞内蛋白含量的差异;甲苯胺蓝染色及番红O染色检测各组软骨细胞合成蛋白多糖的能力差异。结果在黄芪甲苷浓度为5～40μg/m l范围内,药物对软骨细胞增殖速度、细胞活性、细胞内蛋白含量以及细胞异染反应起促进作用,药物浓度越大其促进作用越强;黄芪甲苷浓度达到或超过80μg/m l时,药物对其起抑制作用。结论黄芪甲苷具有促进软骨细胞增殖、合成细胞内蛋白以及蛋白多糖等作用,这种作用在40μg/m l含药浓度时最强。
Objective 1. To study the effect of Astragaloside A on the ability of cartilage cell proliferation , in order to provide a new way for cartilage tissue engineering to obtain a large number of seed cells in a short-term. 2. To study the effect of Astragaloside A on the ability of cartilage cell in synthetizing protein and proteoglycan within the cell , to investigate the impact of Astragaloside A on physiologieal function of euhured cartilage. Methods The cartilage cells of New Zealand White Rabbits were vaeeinated in medium having Astragaloside A with different eoncentration（ 5 μg/ml, 10 μg/ml, 20 μg/ml,40μg/ml, 80 μg/ ml, 160 μg/ml ） , Counting method was used to detect the differences in cartilage cell proliferation among these group; MTT assay was adopted to test the cartilage cell activity differentee ;Commassie blue staining was used to deteete protein eontent difference ;Toluidine blue staining and Fan Hong 0 staining were used to deteete proteoglyean synthesis difference. Results Astragaloside A promoted cartilage cell proliferation,intensified eell activity and increased protein content with the concentration from 5 μg/ml to 40 μg/ml. The greater the concentration, the higher the protein content. However, Astragaloside A with the concentration of 80 μg/ml had opposite Results, that is, inhibiting effect. Conclusion Astragaloside A can promote cartilage eell proliferation as well as protein and proteoglycan synthesis ,40 μg/ml concentration shows the strongest effect.
出处
《实用医院临床杂志》
2009年第4期44-47,共4页
Practical Journal of Clinical Medicine
基金
四川省科技厅重大资助项目(编号:308)
