摘要
目的探讨不同电针刺激方案对大鼠脊髓损伤植入脐血干细胞后生长相关蛋白(GAP-43)、碱性成纤维细胞生长因子(bFGF)、胰岛素样生长因子(IGF)表达的影响。方法将192只清洁级SD大鼠用NYU打击器制成T10-11段脊髓损伤模型,造模成功后随机分为:A组为运动区头皮表面投影区电刺激组(A1组电刺激+脐血干细胞移植,A2组只进行电刺激);B组为损伤局部电刺激组(B1组电刺激+脐血干细胞移植,B2组只进行电刺激);C组为运动区头皮表面投影区电刺激+局部电刺激组(C1组电刺激+脐血干细胞移植,C2组只进行电刺激);D组为损伤模型组(D1组移植脐血干细胞不进行电刺激,D2组不移植不进行电刺激)。各组分别于1周、2周、3周、4周、8周、12周取材,采用免疫组织化学荧光染色检测生长相关蛋白(GAP-43)、碱性成纤维细胞生长因子(bFGF)、胰岛素样生长因子(IGF)的阳性表达。结果在各时间点上,电刺激均能提高生长活性物质GAP-43、bFGF、IGF水平;头针加体针的影响大于单纯头针或体针;干细胞移植后水平更高。结论电刺激与干细胞移植对脊髓损伤后微环境均有有利影响,并有协同作用;头针加体针优于单纯头针或体针。
Objective To explore the effects of electrical stimulation in different acupoints on the expression of growth associated protein-43 （GAP-43）, basic fibroblast growth factor （bFGF）, insuliwlike growth factor （IGF） in spinal cord injured rats after the human umbilical cord blood stem cells transplantation. Methods 192 SD rats were injured at T10-11 level with NYU, and divided randomly into groups： Group A received electric stimulation in the scalp of motor area （A1 group received electric stimulation and transplantation of umbilical cord blood stem cell, the rats of A2 group only received electric stimulation） ; group B received local electric stimulation at damaged site （B1 with electric stimulation and transplantation, B2 only with electric stimulation）; group C received electric stimulation in the scalp of motor area and at damaged site （C1 with electric stimulation and transplantation, C2 only with elec tric stimulation）; D1 received transplantation without electric stimulation, D2 neither with transplantation nor electric stimulation. The expression of GAP-43, bFGF and IGF were detected 1, 2, 3, 4, 8 and 12 weeks after operation. Results Electric stimulation in creased the expression of GAP-43, bFGF, IGF, stimulating scalp and body were more than stimulated scalp or body alone, combined with the stem cells transplantation were more than no transplantation. Cunclusion Both electric stimulation and stem cells transplantation can improve the microenvironment for neural recovery synergistically. Stimulating scalp and body is more effective than alone.
出处
《中国康复理论与实践》
CSCD
2009年第8期717-719,共3页
Chinese Journal of Rehabilitation Theory and Practice
基金
中国博士后科学基金面上项目(20070420405)
中国康复研究中心科研基金(2007-19)
