摘要
采用高效液相色谱（HPLC）法测定了凹叶景天中槲皮素和异鼠李素含量。采用Kromasil C18（250mm×4．6mm，5μm）色谱柱，以乙腈-水-磷酸（43：57：1）为流动相，流速为1．0mL·min^-1，检测波长为360nm。得到槲皮素回归方程为y=74197x＋40608，R^2=0．9998，槲皮素在3．6～18．0μg·mL^-1范围内线性关系良好，平均加样回收率为100．65％，RSD=2．02％；异鼠李素回归方程为y=285344x＋5535．1，R^2=0．9998，异鼠李素在1．06～5．30μg·mL^-1范围内线性关系良好，平均加样回收率为101．99％，RSD=4．95％。该方法简便、高效、准确度较高，结果令人满意，为制定凹叶景天的质量标准及指纹图谱的研究提供了依据。
The contents of quercetin and isorhamnetin in Sedum emarginatum Migo were determined by high performance liquid chromatography （HPLC） using Kromasil C18 （250 min × 4.6 mm, 5 μm） column,with acetonitrile ： water ： phosphoric acid （43 ： 57 ： 1） as mobile phase at a flow rate of 1.0 mL · min^-1 and detection wavelength of 360 nm. The results showed that the regression equation for quercetin was y = 74197x＋ 40608, R^2 =0. 9998, quercetin of 3.6-18.0 μg · mL^-1 had a good linear relationship, the average recovery was 100.65%, RSD=2.02% the regression equation for isorhamnetin was y = 285344x 4-5535.1, R^2= 0. 9998, isorhamnetin of 1. 06- 5. 30μg · mL^-1 had a good linear relationship, the average recovery was 101.99 %, RSD =4.95 %. This method is simple, efficient with high accuracy and satisfactory results, so it can provide reference for the establishment of quality standard and fingerprint spectrum study of Sedum emarginatum Migo.
出处
《化学与生物工程》
CAS
2009年第8期91-94,共4页
Chemistry & Bioengineering
