摘要
白腐真菌的巨大而广谱的生物降解能力使其在"三废"治理和环境修复中具有良好的应用前景。为寻求一种简便有效的白腐真菌总DNA的提取方法,在实验中分别采用蜗牛酶法、CTAB法、SDS法和蛋白酶K法进行操作,且对提取的DNA进行紫外吸收光谱和琼脂糖凝胶电泳(AGE)检测。通过对提取的总DNA浓度、纯度、实验操作过程以及试验成本等方面的比较与分析,得出蜗牛酶法是白腐真菌总DNA提取的理想方法。
White-rot fungi have great prospects of pollutants degradation and environmental rehabilitation which due to their exten- sive biodegradability. In order to find a effective method of extracting total DNA from white-rot fungi, methods like glusulase-exreaction, CTAB-extraction, SDS-extraction and protease K-extraction were used and the extracted DNA were detected with ultraviolet absorption spectrum and agarose gel electrophoresis （AGE）. The comparison and analysis of the concentration, purity quotient of the extracted DNA, operation process and experimental cost showed this glusulase-extraction was the ideal method of extracting total DNA from whiterot fungi.
出处
《生物学杂志》
CAS
CSCD
2009年第4期82-85,共4页
Journal of Biology
基金
安徽省自然科学基金研究项目(No.070413132)
安徽大学人才队伍建设项目
关键词
白腐真菌
总DNA
蜗牛酶法
CTAB法
SDS法
蛋白酶K法
White-rot fungi
total DNA
glusulase-extraetion
CTAB-extraction
SDS-extraction and protease K-extraction
