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沉默Bcl-2基因对A549细胞的影响

Effect of Bcl-2 silencing gene on A549 cell apoptosis
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摘要 目的探讨Bcl-2基因在抑制细胞凋亡的作用。方法利用RNA干扰技术，通过向人非小细胞肺癌A549细胞中导入siRNA表达载体，抑制Bcl-2基因表达，诱导细胞凋亡。结果Bcl-2基因沉默导致A549细胞发生凋亡。结论Bcl-2家族成员之间相互拮抗的关系及由此产生的A549细胞凋亡，为治疗肿瘤提供了一条新的途径。 Objective To explore the suppressive effect of Bcl-2 gene on tumor cell apoptosis. Methods RNA interference technology was used to suppress Bcl-2 gene expression and induce cell apoptosis by introducting siRNA expression vector into A549 cell of human non-small cell lung cancer. Results Silencing of Bcl-2 gene induced A549 cell apoptosis. Conclusions Members of Bcl-2 family are mutually antagonistic, and further induce A549 cell apoptosis, which provides a new strategy to treat tumor.
作者 卢宏志 崔欣 王军 柳艳 高福禄 薛芳 王切
机构地区 秦皇岛市第一医院呼吸内科 保定市第三医院神经内科 三河市中医院内一科 河北师范大学生命科学学院 河北医科大学生物化学与分子生物学教研室 河北医科大学人体解剖学教研室
出处 《国际呼吸杂志》 2009年第16期978-983,共6页 International Journal of Respiration
关键词 BCL-2 细胞凋亡 RNA干扰 Bcl-2 Cell apoptosis RNA interference
分类号 R734 [医药卫生—肿瘤]
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参考文献17

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二级参考文献10

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国际呼吸杂志
2009年 第16期

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