摘要
目的:探讨重组质粒pZeoSV2(+)/CpG-HBcAg(ISS)对BALB/c小鼠免疫的作用。方法:构建真核表达质粒pZeoSV2(+)/CpG-HBcAg(ISSb,c),将其免疫BALB/c小鼠,ELISA法检测免疫后小鼠血清中IFN-γ、IL-2、IL-12、IL-4和IL-10的含量。结果:pZeoSV2(+)/CpG-HBcAg(ISSb)组较pZeoSV2(+)/CpG-HBcAg(ISSc)组免疫BALB/c小鼠后能使Th1型细胞因子IFN-γ、IL-2和IL-12的表达增强,抑制Th2型细胞因子IL-4和IL-10的产生。结论:含CpG-HBcAg(ISSb)的重组质粒能够明显诱导小鼠血清Th1型细胞因子的表达,抑制Th2型细胞因子的表达。
Objective： To explore the immone effects of recombinant plasmid pZeoSV2（ ＋ ）/CpG-HBcAg（ISS） on BALB/c mice. Methods： Recombinant plasmid pZeoSV2（ ＋ ）/CpG-HBcAg（ISSb, c） was constructed and was used to immune BALB/c mice. The serum IFN-γ, IL2, IL-12, IL-4 and IL-10 level were detected by ELISA. Resuits： The IFN-γ, IL2 and IL-12 induced by pZeoSV2 （ ＋ ）/CpG-HBcAg （ISSb）group were obviously higher than those of pZeoSV2 （＋）/CpG-HBcAg（ISSc）group （P（0. 01）, as well as , levels of IL-4 and IL-10 induced by pZeoSV2（ ＋ ）/CpG-HBcAg（ISSb）group were obviously less than those of pZeoSV2（ ＋ ）/CpG-HBcAg（ISSc）group （P〈0. 01）. Conclusions： The result showed that high levels of Thl type cytokines including IL-2, IFN-γ and IL-12 were induced in pZeoSV2（ ＋ ）/CpG-HBcAg（ISSb）recombinant plasmid groups, where as the levels of Th2 type cytokines such as IL-4 and IL-10 were inhibited obviously.
出处
《内科急危重症杂志》
2009年第4期193-196,共4页
Journal of Critical Care In Internal Medicine
基金
湖北省自然科学基金资助项目(No:2006ABA139)
