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多种属CpG ODN诱导特异性抗体反应的研究 被引量：1

Study of specific antibody responses induced by multi-species CpG oligodeoxynucleotides
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摘要 目的探讨人工合成的CpG ODN对不同物种的免疫刺激活性,开发具有广谱免疫刺激活性的CpG ODN。方法将特定的CpG ODN单独或与不同的抗原联合分别免疫鸡和小鼠,用ELISA检测不同时间鸡和小鼠特异性抗体的滴度。结果CpG ODN均能诱导鸡和小鼠产生抗原特异性抗体,且抗体水平明显高于对照组(P<0.05)。结论这种CpG ODN在一定程度上具有广谱免疫刺激特性。 Objective To investigate the immune stimulation activity of CpG oligodeoxynucleotides to different species and develop CpG oligodeoxynucleotides that possess broad - spectrum immune stimulation activity. Method Chickens and mice were respectively vaccinated with CpG oligodeoxynucleotides or synergistically with different antigens, and then titers of specific antibody in the blood serums of chickens and mice were detected with ELISA. Results CpG oligodeoxynucleotides could induce the generation of antigen specific antibody in chickens and mice, and the level of antibodies in groups which use CpG ODN as adjuvant is higher than that in the control group （P 〈 0.05 ）. Conclusion CpG ODN that we made restrictively possesses broad -spectrum immune stimulation activity.
作者 孟民杰 郭晓磊 戴帆
机构地区 广东药学院生命科学与生物制药学院
出处 《广东药学院学报》 CAS 2007年第3期312-314,共3页 Academic Journal of Guangdong College of Pharmacy
基金 广东药学院基金资助(2004SMK18)
关键词 CPG ODN 种属特异性 疫苗 CpG ODN multi-species specificity vaccine
分类号 R392.2 [医药卫生—免疫学]
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参考文献8

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二级参考文献30

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共引文献15

同被引文献31

  • 1张小飞,杨倩.几种黏膜免疫佐剂对鸡小肠IgA分泌细胞的影响[J].畜牧兽医学报,2007,38(1):96-100. 被引量：17
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广东药学院学报
2007年 第3期

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