摘要
目的 动态观察脓毒症时心肌细胞凋亡及Bcl-2和Caspase-3表达的变化，初步探讨脓毒症时心肌细胞凋亡的机制。方法将60只Wistar雄性大鼠随机分为对照组（n=30）、实验组（n=30），两组又分为3、6、12、24及36h共5个亚组。实验组采用盲肠结扎穿孔（CLP）法建立脓毒症模型。对照组行假手术，除不做CLP外，余同实验组。通过TLrNEL法检测心肌细胞凋亡指数（越），免疫印迹法检测Bcl-2及Caspase．3水平的变化。结果（1）CLP术后心肌细胞AJ呈进行性增加，36h达高峰（43．742±2．495），与对照组（4．311±0．035）相比，差异有显著性（P〈0．01），但24h和36h相比，差异无显著性（P〉0．05）。（2）CLP术后心肌组织Bcl-2呈持续下降，36h下降最明显，与对照组相比差异有显著性（P〈0．01），但24h和36h差异无显著性（P〉0．05）。术后心肌组织Caspase-3呈持续上升，36h上升最明显，24h与36h差异无显著性（P〉0．05）。（3）大鼠心肌组织Bcl-2与心肌细胞AJ之间存在负相关（r=-0．753，P〈0．001）。结论细胞凋亡可能是脓毒症心肌损害的机制之一，通过干预心肌细胞凋亡有望改善脓毒症的预后。
Objective To investigate myocardial apoptosis in the septic rats and to evaluate the role of Bcl-2 ,Caspase-3 in signal transduction of myocardial apoptosis. Methods Sixty Wistar rats were randomly divided into control group （n =30） and septic group （n =30）. Either group was then divided into 5 subgroups：3 h,6 h,12 h,24 h and 36 h subgroup. Sepsis model was established by cecal ligation and puncture （CLP）. Control group were given the same treatments except CLP. TUNEL method was used to determine apoptosis index （AI）. Western blot was applied to determine Bcl-2 and Caspase-3 expression. Results （ 1 ） AI value increased progressively after CLP procedure and peaked at 36 h（43. 742 ± 2. 495） ,which showed significant difference compared with control group（4. 311 ± 0. 035 ）. There was no significant difference between 24 h and 36 h subgroup （P〉0. 05）. （2） Bcl-2 protein levels significantly decreased after CLP procedure compared with control group （ P 〈 0. 01 ） and 36 h subgroup was the lowest,but there was no significant difference between 24 h and 36 h subgroup （ P 〉 0. 05 ）. Caspase-3 levels gradually increased after CLP procedure,and 36 h subgroup was the highest,but there was no significant difference between 24 h and 36 h subgroup （ P 〉 0. 05 ）. （ 3 ） There was negative correlation between Bcl-2 and AI （ r = - 0. 753, P 〈 0. 001 ）. Conclusion Myocardial apoptosis may be one of mechanisms for myocardial injury in sepsis. Anti-apoptosis intervention may be a promising therapy to improve the prognosis of sepsis.
出处
《中国小儿急救医学》
CAS
2009年第4期355-357,361,共4页
Chinese Pediatric Emergency Medicine
基金
基金项目：辽宁省自然科学基金[辽科基发（2005）8号]
