摘要
采用温室苗期模拟NaCl盐分胁迫方法,对来自于21个国家的偃麦草属8个植物种34份种质材料苗期的叶片相对含水量(RWC)、相对电导率(REC)、脯氨酸(Pro)、K+/Na+、相对生长速度变化率、耐盐系数、存活率和出现盐害到死亡时间等指标进行测定,且将0.9%NaCl浓度下的各项生理指标变化率和生长指标测定值,利用欧氏最大距离聚类分析,将34份偃麦草属植物种质划分为3个耐盐群体(级别):即耐盐种质、中度耐盐种质、敏盐种质。在此基础上,对NaCl胁迫下的RWC、REC、Pro和K+/Na+等生理指标进行比较分析,结果显示:随着NaCl胁迫浓度的增加,不同耐盐群体的偃麦草属植物种质材料叶片的各项生理指标表现出不同的变化趋势,其中REC、Pro、Na+均呈逐渐增加趋势,而K+、K+/Na+和RWC则呈逐渐下降趋势,但当NaCl浓度上升至1.2%时,与对照(CK)相比,耐盐种质的REC、Pro和Na+分别增加了121.83%、29.25倍和145.45%,K+、K+/Na+和RWC分别下降了26.89%、74.26%和16.40%;而敏盐种质的REC、Pro和Na+分别增加了199.20%、75.45倍和617.86%,K+、K+/Na+和RWC分别下降了43.16%、93.55%和27.69%,中度耐盐种质的增加和下降幅度居于二者之间。
The simulating salinity stress method in green house was adopted, and the relative water content, the relative electrical conductivity rate（REC）, the content of free proline（Pro）, K^＋/Na^＋ , the change rate of relative growth （RWC）, the salt-tolerant coefficient, the livability and the days of salinity injure to death of 34 germplasm and materials of 8 species of Elytrigia Desv. collected from 21 different countries were measured and analyzed at seedling stage, and the above 8 index values at 0.9% NaCl concentration were analyzed using the Euclidean Farthest Distance Cluster Method. The results showed that 34 germplasm and materials were classified into 3 salt tolerant colonies （classes）, namely higher salt tolerant germplasm （HSTG）, medium salt tolerant germplasm （MSTG） and sensitive salt germplasm （SSG）. And different variation trends and rules of the physiological indexes of different salt tolerant Elytrigia Desv. germpalsm were showed, among them, REC, Pro and Na＋ showed gradually increase but K＋ ,K^＋/Na^＋ and RWC showed gradually decrease, but when NaCl concentration was raised up to 1.2%, REC, Pro and Na^＋ were increased by 121.83%, 29.25 times and 145.45% ; K^＋ , K^＋/Na^＋ and RWC were decreased by 26.89%, 74.26% and 16.40% for the HSTG, respectively. However, REC, Pro and Na^＋ were increased by 199.20%, 75.45 times and 17.86%; K^＋ , K^＋/Na^＋ and RWC were decreased by 43.16%, 93.55% and 27.69% for the SSG, respectively. The increase and decrease scales of the MSTG were in the middle.
出处
《干旱地区农业研究》
CSCD
北大核心
2009年第4期83-89,共7页
Agricultural Research in the Arid Areas
基金
国家自然科学基金项目(30571321)
"十一五"国家重点科技支撑项目(2008BADB3B05)
北京市农林科学院常规育种财政专项项目
北京市科技计划项目(Z09090501040902)
关键词
偃麦草属
种质
耐盐性
生理指标
Elytrigia Desv.
germplasm
salt tolerance
physiological indexes
