摘要
目的：建立白及药材中双[4-（β-D-吡喃葡萄糖氧）苄基]-2-异丁基苹果酸酯的含量测定方法，为其质量控制提供科学依据。方法：采用HPLC直接测定，色谱柱为Symmetry C18柱，保护柱为SecurityGuard C18柱，流动相为甲醇-水（37：63），检测波长223nm。结果：测定了12批不同产地市售的白及药材，双[4-（β-D-吡喃葡萄糖氧）苄基]-2-异丁基苹果酸酯的含量在1．45％～3．32％。结论：该法方便、快速、准确、重复性好，可以控制该药材的质量。
Objective： To develop an HPLC method for determination of militarine in Rhizoma Bletillae. Method： Analysis was carried out on a Symmetry C18 analytical column, and a C18 guard column eluted with mobile phase consisted of acetonitrile and water （37：63 ） militarine the detection wavelength was set at 244 nm. Result： The contents of in 12 samples of Rhizoma Bletillae in market were in the ranges of 1.45 % -3.32%. Conclusion： The method is simple, accurate and reproducible, and is suitable for the quality control of Rhizoma Bletillae.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第16期2076-2078,共3页
China Journal of Chinese Materia Medica
