摘要
目的:研究不同生物反应器对丹参不定根培养的影响。方法:利用组织培养技术结合高效液相色谱法,考察不同生物反应器对丹参不定根生长以及丹参不定根有效成分丹参酮ⅡA(TA)及原儿茶醛(PA)含量积累的影响。结果:不同反应器培养丹参不定根中,60°圆锥型反应器最有利于丹参不定根有效成分的积累。测定60°圆锥型反应器中的丹参不定根生长曲线以及有效成分产物积累曲线形如"S"型。丹参不定根培养在第35天达到最大生物量为16.24 g.L-1(鲜重),有效成分丹参酮ⅡA(TA)和原儿茶醛(PA)在第40天达到最高,分别为0.23,0.51 mg.g-1干重。结论:60°圆锥型反应器最有利于丹参不定根有效成分的积累,本实验对于丹参不定根大规模培养具有潜在意义。
Objective： To study the affects of the different bioreactors on the Salvia miltiorrhiza adventitious root culture. Method ： Adventitious roots of S. miltiorrhiza were induced and in vitro cultured in bioreactors. The type of bioreactors was optimized and the kinetics of root growth, accumulation of the active ingredients was also investigated by HPLC. Result： It showed that the 3-1 conical bubble bioreactor （CNBB） with 60° taper favors achieved the highest active compounds accumulation in adventitious roots. The growth curve and secondary metabolites accumulation curve looks like ＂S＂ in CNBB bioreactor. The maximum adventitious roots biomass of 16.24 g · L^-1 （fresh weight） was obtained at 35 day. The highest content of tanshinone Ⅱ A （TA） and protocatechuic aldehyde （PA） reached 0.23 mg· g ^-1 DW and 0. 51 mg · g^-1 DW at 40 day, respectively. Conclusion： The 3-1 conical bubble bioreactor （CNBB） with 60° taper favors was the best bioreactors for the accumulation of active compounds.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第16期2027-2030,共4页
China Journal of Chinese Materia Medica
基金
国家高技术研究发展计划(863)项目(2003AA2Z2040)
国家中医药管理局中医药科学技术研究专项项目(04205ZP10)
关键词
不定根
有效成分积累
生物反应器
丹参
adventitious roots
accumulation of the active ingredients
bioreactor
Salvia miltiorrhiza
