摘要
目的：建立皮革样品中富马酸二甲酯残留量的UPLC测定方法和样品前处理方法。方法：样品经乙腈提取后,ENVI-Carb固相萃取柱和中性氧化铝固相萃取柱净化,进行UPLC定量分析。试验采用BEH C18色谱柱,以水和乙腈为流动相。结果：方法测定低限为0.1 mg/kg,在0.1-5 mg/kg添加水平范围内,富马酸二甲酯回收率为71.9%-80.1%,相对标准偏差为2.5%-4.6%。结论：方法准确度和稳定性较好,操作简单快捷,样品净化效果好,测定低限能够满足限量要求。
Objective： A UPLC method for the determination of dimethy fumarate residue in leathers has been develoed.Methods：Dimethy furmarate was extracted from sample with acetonitrile and cleaned up with ENVI-Carb SPE cartridge and ALUMINA N SPE cartridge;The analyte was determinated by UPLC for the quantitative determination,and quantified by the external standard curve.The separation was performed on a UPLC BEH C18 column with a system of water-acetonitrile as mobile phase at a flow rate of 0.3 ml/min.Results：The limit of determination of this method was 0.1 mg/kg.The detection limits of 0.15 mg/kg proved to be much better than the previously reported ones.Average recoveries of Dimethy fumarate ranged from 71.9% to 80.1%,with relative standard deviations between 2.5%～4.6%.Conclusion：The method is simple,accurate and suitable for the identification and quantification of dimethy fumarate residue in leathers.
出处
《中国卫生检验杂志》
CAS
2009年第8期1801-1803,共3页
Chinese Journal of Health Laboratory Technology
关键词
超高效液相色谱
皮革
富马酸二甲酯
残留
测定
Ultra performance liquid chromatography
Leathers
Dimethy fumarate
Residue
Determination
