摘要
在碱性介质中，铜氨络离子会催化过氧化氢与土霉素、甲烯土霉素或强力霉素的化学发光反应，表面活性剂溴化十六烷基三甲基铵（ＣＴＭＡＢ）对该反应具有增敏作用．发光强度与土霉素、甲烯土霉素或强力霉素的含量分别在８．０×１０－８～８．０×１０－６ｍｏｌ／Ｌ、５．０×１０－８～５．０×１０－６ｍｏｌ／Ｌ和１．０×１０－７～１．０×１０－５ｍｏｌ／Ｌ的范围内分两个量程呈线性关系．７次平行测定的相对标准偏差分别为１．８％～４．１％、２．４％～３．５％和２．５％～４．０％．标准加入的回收率为９９．６％～１０１．４％、９９．１％～１０２．３％和９９．４％～１０１．４％．应用于尿液中这３种四环素类药物的回收测定。
The chemiluminescence reactions of oxytetracycline ( OTC ), methacycline ( MC) or deoxycycline(DC) with hydrogen peroxide were catalysed by the [Cu(NH3)4]2+ ion in ammonia medium.The CL intensities were enhanced by CTMAB. The CL intensities were linear with their contents in 8.0×10-8～8.0×10-6, 5.0×10-8 ～5.0×10-6 and 1.0×10-7～5.0×10-5 mol/L for OTC,MC and DC respectively. The relative standard deviations for 7 determinations were from 1.8～4.1,2.4～3.5 and 2.5～4.0 % for OTC,MC and DC respectively. The recoveries were from 99.6～101.4,99.1～102.3 and 99.4～101.4 % for OTC,MC and DC respectively . This method can be used to the determinations of OTC,MC and DC in urine with satisfactory results.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期547-552,共6页
Journal of Xiamen University：Natural Science
基金
福建省自然科学基金
