摘要
研究了转植物CaM基因的大肠杆菌的培养以及从该工程菌中分离纯化CaM的方法，观察到在含氨节青霉素及执菌素的LB液体培养基中，在35℃培养至在600nm的光吸收为0．5～0．7后，收集菌体破碎，并经苯基琼脂糖疏水层析柱亲和层析，可得到高纯度的CaM，其最高得率为每升菌液得到20mg的CaM．
The methods for the culture of transgenic E. colt and purification of plant:almodulin from the above engineering bacteria were investigated. It was shown that the3unable density of engineering bacteria as judged by A,,, is from 0. 5-0. 7,as the engineering bacteria was cultured in LB liquid medium containing 100 mg/L kanamycin and25 mg/L chloramphenicol. The calmodulin in the engineering bacteria was purified byphenyl-sepharose 4B affinity chromatography and the purity was judged by SDS PAGE,about 20 mg of purified calmodulin was able to be purified from 1 liter of the engineering bacteria.
出处
《河北师范大学学报（自然科学版）》
CAS
1998年第3期389-391,共3页
Journal of Hebei Normal University：Natural Science
基金
国家自然科学基金
