摘要
目的应用表面增强激光解析电离飞行时间质谱(SELDI-TOF MS)技术探讨大鼠肾脏缺血/再灌注(I/R)损伤后血清中纤维蛋白原的变化。方法制备大鼠肾脏I/R模型,分为再灌注6,12,24h组。选取各组血清样本,经SELDI-TOFMS分析检测,并对各组具有差异的多肽指纹图谱鉴定分析并用Proteinchip Software3.1进行校正。使用基质辅助激光解析电离飞行时间质谱(MALDI-TOF/TOFMS)进行蛋白质数据库检索以确定其性质,并使用ELISA的方法检测其在各组中的含量。结果①血清经SELDI-TOFMS分析后,在m/z为2081D处,检测得到具有统计学意义的蛋白差异峰;②采用蛋白质数据库检索,鉴定为大鼠纤维蛋白原片段;③ELISA测得纤维蛋白原在各组大鼠血浆中含量的变化与SELDI-TOFMS检测结果基本一致。结论纤维蛋白原在肾脏缺血/再灌注损伤中可能起重要作用。
Objective To investigate the changes of fibrinogen in renal ischemia/reperfusion（I/R） injury rats using surface enhanced laser desorption/ionizayion time of flight mass spectrometry（ SELDI-TOF MS） technology. Methods After establishing renal ischemia/ reperfusion rat model, rats were divided into three groups：6 h, 12 h, 24 h reperfusion groups. The serum samples were chosen for the test. The peptide fingerprints which existed differences in each group were analyzed by SELDI-TOF MS technology and rectified by Proteinchip Software3.1. Their characteristics were confirmed with MALDI-TOF/TOF MS by searching the protein database,and their contents were determined using ELISA in each group. Results After the serum was detected by SELDI-TOF MS,the statistically significant peptide fingerprints were identified in the m/z 2 481 D. By searching the protein database, they were identified as rat fibrinogen fragments. The change of fibrinogen contents which was detected by ELISA was in accordance with the results detected by SELDI-TOF MS. Conclusion Fibrinogen may play an important role in the renal ischemia/reperfusion（I/R）injury.
出处
《山西医科大学学报》
CAS
2009年第8期685-687,共3页
Journal of Shanxi Medical University
基金
山西医科大学第二临床医学院博士启动基金资助项目(2006-15)
