摘要
目的:观察10味中药对α-葡萄糖苷酶的抑制作用,筛选对α-葡萄糖苷酶抑制作用较强的中药。方法:提取大鼠小肠α-葡萄糖苷酶,以麦芽糖为底物,筛选10味中药水提取物体外对α-葡萄糖苷酶活性的抑制作用;选择体外对α-葡萄糖苷酶活性有较强抑制作用的中药提取物,一次性灌胃后对餐后60min血糖的影响;以阿卡波糖(ACAR)作为阳性对照药物。结果:赤芍、山茱萸和桑白皮3味中药对α-葡萄糖苷酶的活性有较强的抑制作用,显示明显的浓度-效应关系,其中桑白皮抑制作用最强,当桑白皮浓度达到10mg/ml时,其对α-葡萄糖苷酶的抑制率已达80%,相当于浓度1mg/ml的ACAR的作用;3种中药体内实验结果表明,3者均可抑制大鼠一次性灌胃麦芽糖60min后血糖水平的升高(P<0.05或P<0.01),与对照组比较,对血糖升高的抑制率以桑白皮组最高(62.09%),并且与ACAR(69.31%)组没有统计学差异(P>0.05)。结论:赤芍、山茱萸和桑白皮3味中药体内、外对α-葡萄糖苷酶活性均有较强的抑制作用。
Objective： To observe the inhibitory effect on a-glucosidase of 10 kinds Chinese herbs and to screen the Chinese herbal medicines which have great inhibitory effect on α -glycosidase. Methods： The α -glucosidase was extracted from small intestine of rat. The amount of glucose was measured with produced from substrate of malt sugar. The inhibitory effect of 10 kinds of Chinese herbs on α -glucosidase was observed by this enzyme reaction system. Then disposable gastric perfused malt sugar （2 g/kg） and the extraction screened at the same time, detected the levels of blood glucose after 60 rain. The positive control group is acarbose （ACAR） group. Results： The three kinds Chinese herbs （Chishao, Shanzhuyu and Sangbaipi） showed very good inhibitory activities, and they showed obviously concentration-effect curve relationship. Among them, the inhibitary activity of Sangbaipi is stronger than Chishao and Shanzhuyu. While the dose of Sangbaipi reached 10mg/mL, the inhibition rate arrived 80%, which effect was equivalet to the dose of lmg/ml acarbose. The results of postprandial blood glucose in vitro showed us： Sangbaipi, Chishao and Shanzhuyu can inhibit postprandial blood glucose levels in rats that have been disposable gastric perfused malt sugar after 60rain （P〈0.05 or P〈0.01）. Compared with the control group, the inhibit rate of Sangbaipi is the strongest （62.09%） on blood glucose increase and there is no difference to ACAR group （P〉0.05）. Conclusion： The three kinds of Chinese herbs （Chishao, Shanzhuyu and Sangbaipi） can inhibit α-glucosidase activity obviously in both vitro and vivo.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2009年第8期1035-1037,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
中国科技部国际科技合作项目(No.2008DFA31050)
