摘要
采用新科技膜分离与醇沉法结合进行纯化大豆异黄酮,建立大豆异黄酮含量的测定方法,确定膜分离中的技术参数,即膜材料、膜孔数、膜再生、膜通量与操作压力和温度的关系等。用树脂法和醇沉法纯化大豆异黄酮的方法比较,并对醇沉法中许多工艺参数进行了单因素试验,用正交试验确定了最佳工艺参数:以大豆异黄酮含量为考查指标时,其最佳工艺为浓缩液质量分数为30%,按醇料比7:1加入70%乙醇,搅拌10min进行醇沉。上清液回收乙醇后调节其可溶性固形物含量为5%,静置5h,再以2000r/min速度离心10min,对沉淀进行水洗,弃去水洗液,沉淀物60℃干燥,在此条件下可制备大豆异黄酮含量大于60%的产品;以大豆异黄酮得率为考查指标时,醇料比5:1、浓缩液质量分数为30%、回收乙醇后可溶性固形物含量8%、静置4h,其他条件相同,可得大豆异黄酮得率为0.7‰左右。
A new technology of combined membrane separation and alcohol precipitation was applied for the purification of isoflavones from the ethanol extract of low-denatured soybean meal. The method for the determination of isoflavone content was developed, and the membrane separation conditions of isoflavones including membrane material type and membrane hole number, membrane regeneration, and the relationship of membrane flux with operation pressure and temperature were investigated. Compared with resin adsorption method, alcohol precipitation was easier to operate and had less process steps and led to higher ptLdty of isoflavones. Alcohol precipitation parameters for the purification of isoflavones were optimized by single-factor and otthogonal array methods. Under the optimized conditions as follows： mass fraction of isoflavone extract 30%, 70% ethanol/material ratio 7： 1, stirring time 10 min, adjusting soluble solid content in the supemant after recover of ethanol to 5%, and standing time 5 h, the content of isoflavones in final product was above 60%. An isoflavone yield of 0.7%0 was obtained under the optimized conditions as follows： mass fraction of isoflavone extract 30%, 70% ethanol/material ratio 5：1, soluble solid content in the supemant 8%, and standing time 4 h.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第16期78-82,共5页
Food Science
基金
黑龙江省"十五"高技术产业化研发项目(FG-2002001)
"十五"国家现代农业高技术产业化示范工程项目(发改高技[2003]1933号)
关键词
膜分离
醇沉法
异黄酮含量
工艺参数
membrane separation
alcohol precipitation
isoflavone content
technical parameters
