摘要
目的:探讨胎鼠脊髓在成鼠损伤脊髓神经通路修复中的作用.方法:将E14大鼠胚胎脊髓植入成鼠损伤脊髓后30、50、70天时,通过坐骨神经和红核引入CB-HRP,取移植部位脊髓做冰冻切片,经TMB组化显色后,再分步制成电镜标本,然后在电镜下观察移植物和宿主脊髓的纤维联系.结果:术后30天时,来自背根的轴突再生进入移植物,附近运动神经元和中间神经元的突起也在此时向移植物内发送新支.术后50天时,来自背根有的轴突和胚胎神经元形成了以轴-树突触为主的突触连接.与此同时,红核脊髓束的纤维也以相对缓慢的速度向移植物再生.它们于术后70天时与移植胚胎元的树突形成了轴-树突触.结论:胚胎脊髓移植在一定程度上可以恢复损伤脊髓的神经通路.
The fiber connection between the graft and host tissue was traced by CB-HRP under electron microscope 30, 50, and 70 days after E14 embryonic spinal cord being transplanted into acutely injured spinal cord of adult rats. The results were as follows; The axons of the dorsal root regen-crated into the graft 30 days after transplantation and the motoneurons and interneurons sent their processes to the graft by sprouting 50 days after transplantation, dorsal afferent axons formed synaptic connection which was mainly axonal-dendritic synapse with embryonic neurons and meanwhile rubrospinal fibers regenerated at relatively slow speed towards the graft and reformed axonal-dendritic synaptic connection with the fetal neurons 70 days after transplantation. The results suggest that the injured nerve tracts may be reconstructed in some degree by transplanting embryonic spinal cord into traumatized spinal cord.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1998年第4期288-292,共5页
Chinese Journal of Anatomy
基金
国家自然科学基金资助项目
