摘要
目的研究复方TH体内外抗肿瘤活性,筛选抗肿瘤新复方。方法体外采用噻唑蓝(MTT)法检测复方TH对体外培养的人肺癌细胞A549、人食管癌细胞EC9706、人结肠癌细胞LoVo、人肝癌细胞Hep-G2、人乳腺癌细胞MCF-7的生长抑制作用,计算生长抑制率及半数抑制浓度(IC50)。将小鼠接种肝癌H22细胞后随机分成5组:空白对照组(双蒸水)、阳性对照组(顺铂0.005g·kg-1)、3个剂量复方TH给药组(0.030,0.060,0.090g·kg-1),复方TH组次日开始灌胃给药0.3mL,连续8d。每间隔1d每只小鼠腹腔注射给药0.2mL,共3次。末次给药后24h处死小鼠,计算肿瘤抑制率、胸腺指数、脾脏指数。结果复方TH可以显著抑制人肺癌细胞A549、人食管癌细胞EC9706、人结肠癌细胞LoVo、人肝癌细胞Hep-G2、人乳腺癌细胞MCF-7的生长,IC50分别为7.15,5.41,2.49,2.18和1.37μg·mL-1;TH复方小、中、高剂量组的肿瘤抑制率分别为52.8%,44.9%和47.9%;与对照组比较均差异有极显著性(均P<0.01),脏器指数及质量与对照组比较均差异无显著性,与阳性对照组比较差异有极显著性(P<0.01)。结论复方TH对体外培养的肿瘤细胞有较强的细胞毒性作用,可显著抑制细胞生长;体内可以明显抑制实体瘤生长。
Objective To study the anti-tumor effect of TH in vitro and in vivo and identify a new high-efficient and low-toxic compound recipe. Methods Inhibition rate of growth and half-inhibitory concentrations （ IC50 ） were assessed in human lung cancer cell A549, human esophageal carcinoma cell EC9706, human colon cancer cell LoVo, human hepatocellular carcinoma cell tlep-G2 and human breast cancer ceils MCF-7 by MTT method. The mice transplanted with hepatoma H22 were randomly divided into 5 groups： negative control, positive control （ CDDP0. 005 g · kg^-1 ）, three doses of TH（0. 030,0. 060, 0. 090 g · kg^-1 ）, which were treated intragavagely with 0.3 mL of solution since the next day, for consecutive 8 d. Other groups were treated with 0. 2 mL every other day for total three times. Mice were sacrificed 24 h after the last drug administration the indexes of thymus, spleen and inhibitory rates were measured. Results IC50 of TH in A549, EC9706, LoVo, Hep-G2 and MCF-7 were 7.15,5.41,2.49,2.18 and 1.37 μg·mL^-1,respectively. Tumor inhibitory rates of low, middle and high dose group of TH in mice with H22 were 52.8% ,44.9% and 47.9% ,which were significantly higher than those of the control group （P 〈 0. 01 ）. The indexes of spleen,thymus and liver in TH groups showed no remarkable changes compared with those in the control group, but were significantly different from those in the positive control （P 〈 0.01 ）. Conclusion TH has strong anti-tumor activities by markedly inhibiting cell growth in vitro and suppressing tumorigenesis in vivo.
出处
《医药导报》
CAS
2009年第8期996-999,共4页
Herald of Medicine
基金
中国医学科学院放射医学研究所青年基金(基金编号:ST0646)
关键词
TH
复方
抗肿瘤作用
TH, Compound recipe
Anti-tumor activity
