摘要
目的建立测定辛夷挥发油软胶囊中α-蒎烯、β-蒎烯、柠檬烯和1，8-桉叶素的测定方法。方法采用毛细管气相色谱法测定。BP-5毛细管柱（30m×0．25mm，0．22μm）；进样器温度180℃；FID检测器温度200℃；柱温采用程序升温；分流比1：30；苯乙酮为内标。结果α-蒎烯在0．054～2．505μg线性关系良好，平均加样回收率为98．6％，RSD为0.72％（n=9）；β-蒎烯在0．070～3．204μg线性关系良好，平均加样回收率为99．0％，RsD为0．65％（n=9）；柠檬烯在0．068～3．190μg线性关系良好，平均加样回收率为101．1％，RSD为0．56％（n=9）；1，8-桉叶素在0．301～10．205μg线性关系良好，平均加样回收率为99．6％，RSD为1．24％（n=9）。结论该方法简便、准确、重现性好，可作为辛夷挥发油软胶囊的质量控制方法。
Objective To develop a method for determination of α-pinene, β-pinene, limonen, and 1, 8- cineole in FlosMagnoliae volatile oil soft capsula. Methods The contents of α-pinene, β-pinene, limonen, and 1, 8-cineole were determined at the same time by capillary gas chromatography with sequential increase of temperature programming. Capillary column BP-5 （30 m × 0.25 mm, 0. 22 μm） was used, injector temperature 180 ℃, flame ionization detector （FID） temperature 200 ℃, split ratio 1 ： 30, acetophenone as internal standard. Results A good linear relationship of a-pinene was at a range of 0. 054--2. 505 μg, the average recovery was 98.6%, and RSD was 0.72 % （n=9）; A good linear relationship of β-pinene was at a range of 0. 070--3. 204 μg, the average recovery was 99.0 %, and RSD was 0.65% （n=9） ; A good linear relationship of limonen was at a range of 0. 068--3. 190 μg, the average recovery was 101.1%, and RSD was 0.56% （n=9） ; A good linear relationship of 1, 8-cineole was at a range of 0. 301--10. 205 μg, the average recovery was 99. 6%, and RSD was 1.24% （n=9）. Conclusion The method is simple and accurate one with good reproducibility and could be used for the quality control of Flos Magnoliae volatile oil soft capsula.
出处
《中草药》
CAS
CSCD
北大核心
2009年第8期1232-1235,共4页
Chinese Traditional and Herbal Drugs
基金
国家"973"项目(2006CB504707)
