摘要
目的:探讨辛夷挥发油(the Volatile Oil of Magnolia biondii pamp.)对哮喘气道炎症的作用。方法:筛选合格雄性豚鼠30只,体重(180±20)g,随机分为正常对照(A)组、模型对照(B)组、阳性对照(C)组、辛夷挥发油大剂量(D)组和辛夷挥发油小剂量(E)组。A组以生理盐水代替抗原进行致敏和激发。其余各组每鼠后肢im卵白蛋白(OVA)并ip百日咳疫苗致敏并雾化OVA激发,建立哮喘模型。在激发引喘的同时,记录引喘潜伏期。自致敏第2 d始ig给药,1次/d,连续14 d。于激发后24 h处置动物,采制标本与指标检测。结果:(1)首次激发后,引喘潜伏期(s)D组(76.83±14.97)与B组(43.50±7.28)比较,差异有统计学意义(P均<0.01);二次激发后,D组(68.80±31.63)、E组(56.00±23.02)与B组(49.00±10.34)比较,有延长趋势,但差异无统计学意义(P均>0.05),以D组延长明显。(2)D、E组BALF中Eos比值分别为(9.77±7.46)%、(10.67±8.10)%,与B组(15.00±3.00)%比,差异均有统计学意义(P均<0.05)。D组BALF蛋白含量、WBC计数分别为(0.15±0.07)×109/L、(0.17±0.02)×109/L、与B组比,有减少趋势,但差异无统计学意义(P均>0.05)。(3)病理检查和图像分析检测结果,D、E组豚鼠的细支气管粘膜、腔内浸润的Eos数(个/视野)分别为(26.75±5.33)、(50.38±15.18),与B组(79.63±16.65)比明显减少,差异有统计学意义(P<0.01、<0.05),以D组减少显著。D、E组细支气管腔内浸润炎细胞核的积分光密度(IOD)分别为(4.15±3.65)、(4.27±4.09),与B组比有减少趋势,但差异无统计学意义(P>0.05)。结论:辛夷挥发油能够明显抑制实验性哮喘豚鼠气道浸润Eos细胞数,减轻哮喘气道的炎症反应。
Objective： To explore the effect of the Volatile Oil of Magnolia biondii pamp （VOMbP） on airway inflammation in asthmatic guinea pigs. Methods： 30 qualified male guinea pigs were selected, （body weight 180 ± 20 g） and randomly divided into normal control group （group A）, model group （group B）, positive control group （group C）, high dosage of VOMbP group （group D） and low dosage of VOMbP group （group E）. Guinea pigs were allergized and stimulated in order to lead to asthma by means of NS （not antigen）, model were established in other groups by im OVA, and ip Pertussis vaccine was used to allergize and stimulate guinea pigs. while allergizing and stimulating lead to asthma, incubative stage was recored. Asthmatic guinea pigs were administrated by ig 2 days after sensitization, once a day, for 14 days. Animals were killed 24 h after timulating, the index was detected, image analysis were carried out. Results： ①After first sthnulation, the incubation period of group D （76.83 ± 14.97） was prolonged compared with group B （43.50 ± 7.28）, with a significant difference （P 〈 0.01） ; After second challenge, the incubation period of group D （68.80 ± 31.63） and group E （56.00 ± 23.02） tended to be prolonged compared with group B （49.00 ± 10.34）, but without significant difference （P〉0.05）,②The Eos ratio of group D （9.77±7.46）%and group E in BALF （10.67±8.10）% tended to decrease comparing with group B （15.00 ± 3.00）%, but without significant difference （P 〉0.05）. Protein level in BALF [ （0.15 ± 0.07） ×10^9/L] and the blood count [ （0.17 ± 0.02） × 10^9/L] of group D are tended to decrease comparing with group B, without significant difference （P〉0.05）. （3） The detection result of pathology and image analysis： The Eos count in mucous membrane and lumen of bronchiole of group D （26.75 ± 5.33） and group E （50.38 ± 15.18） have significant difference （P〈0.01, 〈0.05） comparing with group B （79.63 ± 16.65）. The infiltration of inflammatory cell in bronchial lumen （IOD） of group D （4.15 ± 3.65） and group E （4.27 ± 4.09） tended to decrease compared with group B, but without significant difference （P 〉 0.05）. Conclusion： OMbP can obviously reduce the cell count of EOS in experimental asthma airway, and alleviate the airway inflammatory reaction in asthma.
出处
《现代预防医学》
CAS
北大核心
2006年第8期1338-1341,共4页
Modern Preventive Medicine
关键词
辛夷挥发油
气道炎症
支气管肺泡灌洗液
嗜酸性粒细胞
Volatile Oil of Magnolia biondii pamp
Airway inflammatory
Bronchoalveolar lavage fluid eosinophile granulocyte
