摘要
目的建立壮元益生丸中松果菊苷和毛蕊花糖苷的含量测定方法。方法采用高效液相色谱法,以YMC-PackODS-A(250mm×4.6mm,5μm)色谱柱为固定相,乙腈-甲醇-1%乙酸水溶液(10:13:77)为流动相,柱温为30℃,流速为1.0ml·min-1,检测波长为334nm。结果松果菊苷的测定在0.168～1.008μg内、毛蕊花糖苷在0.072～0.432μg内与峰面积呈良好线性关系,相关系数均分别为0.9997和0.9996;平均回收率分别为100.25%和100.70%;RSD为1.22%(n=6)和1.41%(n=6)。结论方法简便、准确,重复性好,可用于壮元益生丸的质量控制。
Objective To establish a method for determination echinacoside and acteoside in Zhuangyuan Yisheug Pills by HPLC. Methods The separation was performed on YMC-PackODS-A column. The mobile phase consisted of CH3CN-MeOH-1%HAc （10：13：77）and the detection wavelength was 334 Bin with a PDA detector,The flow rate Was 1.0mL·min^-1 and the column temperature was 30℃.Results The lin- ear ranges of echinacoside and acteoside were 0.168-1.008μg（r=0.999 7） and 0.072-0.432μg（r=0.999 6）,respectively.the average recoveries （n =6） of echinacoside and acteoside were 100.25% （RSD =1.22% ） and 100.70%（RSD=1.41%）,respectively.Conclusion The method was simple, accurate and reproducible.It can be used for the quality control of Zhuangyuan Yisheng Pills.
出处
《中国现代药物应用》
2009年第15期14-16,共3页
Chinese Journal of Modern Drug Application
