摘要
目的建立血栓通胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1含量测定方法。方法采用UltimateTM XB C18色谱柱(4.6mm×200mm,5μm);流动相:乙腈-水梯度洗脱(0～20min(15:85),20～21min(40:60),21～30min(15:85));柱温:30℃;流速:1.0mlomin-1;检测波长:302nm。结果三七皂苷R1的线性范围为0.35～8.68μg(r=0.9996),平均回收率为100.2%(RSD=1.31%);人参皂苷Rg1的线性范围为1.35～33.83μg(r=0.9999),平均回收率为99.50%(RSD=1.08%);人参皂苷Rb1的线性范围为1.71～42.75μg(r=0.9998),平均回收率为99.84%(RSD=1.19%)。结论该方法准确可靠、简单快速,可用于血栓通胶囊质量控制。
Objective To establish an HPLC method for content determination of Xueshuantong capsules. Method The separation was performed with an UltimateTM XB C18 column （4.6 min×200 mm,5 μm） and the mobile phase was acetonitrile-water in linear gradient elution（0-20 min（15：85）, 20-21 min （40：60） , 21-30 min （15：85））. The flow rate was 1.0 mlomin-1 and the wavelength of detection was at 203 nm. The column temperature was 30 ℃. Results The liner range of notoginsenoside R1 was 0.35 - 8.68 μg（ r = 0.999 6） and the average recovery was 100.2 %（ RSD =1.31%）; The liner range of ginsenoside Rg1 was 1.35 - 33.83 μg （r = 0.999 9） and the average recovery rate was 99.50 %（ RSD = 1.08 %）; The liner range of ginsenoside Rbl was 1.71 - 42.75 μg（ r = 0.999 8） and the average recovery rate was 99.84% （ RSD = 1.19%）. Conclusions This method is accurate,simple and can be used to control the quality of Xueshuantong capsules.
出处
《中国现代药物应用》
2009年第15期9-11,共3页
Chinese Journal of Modern Drug Application
