摘要
以一株产纤维素酶的黑曲霉菌株D2为出发菌，进行了微波一亚硝基胍复合诱变，筛选到遗传性状稳定的高产纤维素酶突变菌株N14，其最适生长pHNN5．0～6．0、最适生长温度28℃-30℃，酶解反应的最适pH值为5．0、最适温度50℃。通过单因素和正交试验确定了突变株N14的最佳液态产酶条件是：麸皮为碳源、酵母膏为氮源、碳氮比4：1、起始pH值为6．5，接种量6％，发酵温度30℃，发酵时间3d。在上述条件下，菌株滤纸（FPA）酶活最高，达363．5U／mL，是出发菌株的3．5倍。
The original strain aspergillus niger D2 producing cellulase was treated with microwave irradiation and nitrosoguanidine （NTG）. A mutant NI4 had higher and stable production of cellulase was obtained. The optimum pH and temperature of the strain were 5.0 - 6.0 and 28 - 30℃, respectively. The optimal pH and temperature of enzymatic hydrolysis reaction were 5.0 and 50℃ respectively. In additions, the optimum liquid fermentation conditions of the mutant N14 were obtained through single factor and orthogonal experiment as followed：carbon source bran,nitrogen source yeast extract, the ratio of carbon and nitrogen 4 ： 1, the initial pH 6. 5, the inoeulum 6% （ v/v）, the culture temperature 30℃, the culture time 3 d. Under these conditions, the FPA enzyme activity of the mutant reached 363.5 U/ml ,which was 2. 5 times higher than that of the original strain D2.
出处
《中国酿造》
CAS
北大核心
2009年第8期53-56,共4页
China Brewing
基金
山西省科技厅攻关项目(041030)
关键词
纤维素酶
微波
亚硝基胍(NTG)
诱变
酶学性质
cellulase
microwave
nitrosoguanidine （NTG）
mutagenesis
enzymatic charac - teristies
