摘要
目的建立灵芝破壁孢子粉中多糖含量的测定方法。方法采用紫外分光光度法,检测波长为625nm,用葡萄糖为对照品,测定灵芝破壁孢子粉中多糖含量。结果不同的提取方法对于多糖的提取率不同,最佳提取方法为沸水浴提取8h。结论本法稳定可靠,简便快捷,适用于灵芝破壁孢子粉多糖含量测定。
OBJECTIVE To develop a method for the determination of polysaccharide content in sporodermbroken Ganoderma lucidum spores. METHODS The contents of polysaccharide in sporoderm-broken Ganoderma lucidurn spores were determined by UV spectorphotometry at 625nm, with glucose being used as the reference substance. RESULTS Extraction rates of polysaccharide due to the extraction methods. Heating in a 100℃ water bath for 8 hours was found to be the optimal extraction method. CONCLUSION This method is dependable, convenient and rapid, can be used as the determination of polysaccharide in broken cellular wall spore of Ganoderma lucidum.
出处
《海峡药学》
2009年第7期69-71,共3页
Strait Pharmaceutical Journal
关键词
灵芝
破壁孢子粉
蒽酮-硫酸法
多糖
Ganoderma lucidum
Sporoderm-broken
Spore
Anthrone-sulfuric acid method
Polysaccharide
