摘要
本文建立一种用自动生化分析仪双试剂法检测血清乙醇脱氢酶（ADH）活性的方法。同时对100例肝功正常者与9例原发性肝癌、10例肝硬化、40例肝炎患者测定血清该酶活性。在反应体系中，NAD+和乙醇浓度分别为10．0mmol／L和25．0mmol／L，缓冲液为pH9．00的0．1mol／L甘氨酸-NaOH溶液。ADH对NAD+和乙醇的Km值分别为1．67mmol／L和3．03mmol／L。批内CV为0．7％～5.4％。肝功正常者血清ADH活性为1．4±1．2U／L。原发性肝癌为11．4±2．5U／L，肝硬化为4．6±1．5U／L和肝炎为5．5±1．6U／L。这些患者血清ADH活性显著高于肝功正常者。原发性肝癌患者血清ADH活性显著高于肝硬化和肝炎患者。这种简便、准确、灵敏的测定ADH活性方法可用于临床常规分析。
It was described an improved method for determination of alochol dehydrogenase (EC 1. 1. 1. 1 ) activityfor Chinese by automatic analyzer, based on conversion of ethanol to acetaldehyde with simultaneous reduction of NAD+ in glycine-NaOH buffer (pH 9.0) at 37℃. The final concentration of NAD+ was 10.0 mmol/L and ethanol 25. 0 mmol/L. The Km for NAD+ in this assay was 1, 67 mmol/L and ethanol 3.03 mmo1/L-Within precisi0n (CV) was from 0. 7% to 5. 4% over the range of alcohol dehydrogenase activity from 1. 3to 30- 0 U/L. The mean activity of alcohol dehydrogenase in healthy controls (1. 4t 1. 2 U/L) was significantly lower (P<0.01) than the mean values in primary hepatic carcinoma (11. 4± 2. 5 U/L), liver cirrhosis (4. 6±1. 5 U/L), and hepatitis (5. 5±1. 6 U/L). The activity in primary hepatic carcinoma was significantly higher (P<0.01) than in liver cirrhosis and hepatitis. Because of its simplicity, accuracy, sensitivity,and requirement of small sample volume, we consider the method suitable for routine use in clinical laboratorles.
出处
《上海医学检验杂志》
CAS
1998年第2期84-86,共3页
Shanghai Journal of Medical Laboratory Sciences
关键词
乙醇脱氢酶
血清
自动生化分析
Alcohol dehydrogenase Serum ADH Automatic biochemical assay
