摘要
羊肚菌多糖分别经热水浸提、Sevag法脱蛋白、透析、乙醇沉淀、DEAE-52纤维素层析和Sephadex G-100凝胶层析等分离纯化过程,得到羊肚菌多糖组分PMEP-1,经鉴定PMEP-1为均一组分,得率为1.58%,相对分子质量43600,旋光度[α]22D为+257.0(C=0.001,H2O)。采用多糖化学结构分析的化学方法(部分酸水解、甲基化分析、高碘酸氧化、Smith降解)和现代仪器分析方法(红外光谱、GC-MS、1H和13C核磁共振)对PMEP-1进行全面结构特征分析,结果发现:PMEP-1是由阿拉伯糖、甘露糖、葡萄糖、半乳糖(摩尔比为1:2.37:4.79:3.09)构成,以α-1,4-葡萄糖残基、α-1,6-半乳糖残基、α-1,2-甘露糖残基及α-1,5-阿拉伯糖残基为主要连接,同时含有α-1,2,6-甘露糖分支、α-1,4,6-葡萄糖分支和β-1,2,6-半乳糖分支的高度分支杂多糖。
The polysaccharide PMEP-1 was isolated from Morchella esculenta （L.） Pers. through hot-water extraction and subjected to Sevag deprotein, dialysis, ethanol precipitation, DEAE-52 cellulose ion-exchange column chromatographic analysis and Sephadex G-100 column chromatographic analysis. This polysaccharide was found to be highly homogeneous with a relative molecular mass of 43600 and a specific rotation of ＋257.0 （C = 0.001, H2O） and the yield reached 1.58%. The chemical structure of PMEP-1 was analyzed by chemical methods （including partial hydrolysis with acid, methylation reaction, periodate oxidation, and Smith degradation） and instrument analysis methods （using IR, GC-MS, ^1H-NMR, and ^13C-NMR）. The polysaccharide PMEP-1 was found to be composed of Ara, Man, Glc and Gal with a molar ratio of 1： 2.37： 4.79：3.09 and be a complex heteropolysaccharide with highly ramified structure, whose main chain was formed by a mixing linkage of α- 1,4-glucose, α-1, 6-galactose, α-1,2-mannose and α-1,5-arabose and whose side chains were attached at α-1,2,6-mannose, α-1,4,6-glucose and α - 1,2,6-galactose.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第15期104-108,共5页
Food Science
基金
西南大学博士基金项目(SWU109005)
云南省省校省院科技合作支持项目(2005YX24)
关键词
羊肚菌
多糖
分离纯化
结构分析
Morchella esculenta （L.） Pers
polysaccharide
isolation and purification
structural analysis
