摘要
目的探讨肿瘤坏死因子（TNF）α-238及TNFα-308基因多态性与动脉硬化性脑梗死的关系。方法运用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）技术检测97例动脉硬化性脑梗死（CI组）及141例正常人（对照组）的外周血TNFα-238、TNFα-308基因多态性AA、AG、GG的基因型。结果CI组TNFα-238GG、GA和AA基因型频率分别为88．66％、11．34％和0；对照组分别为95．74％、4．26％和0。CI组TNFα-238G、TNFα-238A基因频率分别为94．33％、5．67％；对照组分别为97．87％、2．13％。TNFα-238各种基因型频率在CI组与正常对照组之间的差异有统计学意义（均为P〈0．05）。CI组TNFα-308GG、GA和AA基因型频率分别为77．32％、8．25％和14．43％；对照组分别为92．20％、5．67％和2．13％。CI组TNFα-308G、TNFα-308A基因频率分别为81．44％、18．56％；对照组分别为95．04％、4．96％。TNFα-308各种基因型频率在CI组与正常对照组之间的差异有统计学意义（均为P〈0．05）。结论TNFα-238和TNFα-308基因多态性AA、AG、GG基因型与CI有关联。
Objective To investigate the relationship of tumor necrosis factor alpha （TNFα）-238 and TNFα-308 gene polymorphism with atheroselerosis cerebral infarction. Methods In 97 patients with atherosclerosis cerebral infarction （CI group） and 141 healthy people （control group）, polymorphisms of TNF α-238 G/A and TNFα-308 G/A gene in peripheral blood were detected by polymerase chain reaction and restriction fragment length polymorphism （PCR-RELP）. Results In CI group, genotype frequencies of TNF α-238 GG, GA and AA were 88.66%, 11.34% and 0, respectively, gene frequencies of TNFα-238 G and TNFα-238 A were 94.33% and 5.67%. In control group, genotype frequencies of TNFα-238 GG, GA and AA were 95.74%, 4.26% and 0, respectively, gene frequencies of TNFα-238 G and TNFα-238 A were 97.87% and 2.13%. There were significant differences in all frequencies of TNFα-238 G/A gene between two groups （all P〈0.05）. In CI group, genotype frequencies of TNFα-308 GG, GA and AA were 77.32%, 8.25% and 14.43%, respectively, gene frequencies of TNFα-308 G and TNFα-308 A were 81.44% and 18.56%. In control group, genotype frequencies of TNFα-308 GG, GA and AA were 92.20%, 5.67% and 2.13%, gene frequencies of TNFα-308 G and TNFα-308 A were 95.04% and 4.96%. There were significant differences in all frequencies of TNFα-308 G/A gene between two groups （all P〈0.05）. Conclusion TNFα-238 G/A and TNFα-308 G/A polymorphism are associated with relating to cerebral infarction.
出处
《中华生物医学工程杂志》
CAS
2009年第2期98-102,共5页
Chinese Journal of Biomedical Engineering
关键词
颅内栓塞
肿瘤坏死因子Α
基因频率
颅内动脉硬化
多态性
Intracranial embolism
Tumor necrosis factor- alpha
Gene frequency
Intracranial arteriosclerosis
Polymorphism
