摘要
背景与目的:探讨多氯联苯(polychlorinated biphenyl,PCB)对大鼠海马神经元细胞生长发育的影响。材料与方法:取新生Wistar大鼠双侧海马,无菌条件下制备细胞悬液。实验分为对照组和PCB低(1×10-8mol/L)、中(1×10-7mol/L)、高(1×10-6mol/L)3个剂量实验组。运用光镜、免疫组化技术研究PCB对大鼠海马神经元细胞形态学及bcl-2和TGF-β1表达的影响。结果:PCB对大鼠海马神经元细胞的损伤随着PCB剂量的增加呈升高趋势,高剂量PCB组海马神经元细胞结构改变明显,神经元细胞轴突和树突消失仅部分残存,胞体萎缩;PCB能诱导海马神经元细胞bcl-2和TGF-β1的表达,PCB低剂量组bcl-2呈强阳性表达,中剂量组bcl-2表达显著下调,高剂量组bcl-2为阴性表达;低剂量组TGF-β1表达显著下调,中剂量组TGF-β1表达上调;高剂量组呈阴性,而对照组神经元细胞的bcl-2和TGF-β1均为阳性表达。结论:PCB对大鼠海马神经元细胞的生长产生影响。
BACKGROUND AND AIM： To study the effects of polychlorinated biphenyl（PCB） on hippocampal neuron cells in rats. MATERIALS AND METHODS： Bilateral hippocampi were isolated from neonatal Wistar rats, cellular suspensions were prepared under sterile conditions. Rats were divided into control group and three experimental groups, the dosage of PCB were low （1× 10^-8 mol/ L ）, middle（1× 10-7 mol/ L） and high （1× 10^-6 mol/ L）, respectively. Light microscope and immunohistochemical technology were used to examine the effects of PCB on structure and function of hippocampal neuron cells in rats. RESULTS： The structural damage of hippocampal neuron cells was related to the dose of PCB, neuron cells were most seriously affected in the high dose group. Axons and dendrites disappeared to remnants, with atrophy of cell bodies. PCB could induce bcl-2 and TGF-β1 expressions in hippocampal neuron cells, bcl-2 expression was detected in control, low dose and middle dose groups, strongest in the low dose group, while bcl-2 expression was not found in the high dose group. TGF-β1 expression was positive in control group. PCB could inhibit TGF-β1 expression in low dose group , but the expression was up-regulated by PCB in middle dose group. CONCLUSION： PCB could affect the development of hippocampal neuron cells.
出处
《癌变·畸变·突变》
CAS
CSCD
2009年第4期298-301,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
甘肃省自然科学基金资助项目(ZR-97089)
