摘要
背景与目的:探讨多氯联苯(polychlorinated biphenyl,PCB)对大鼠肝脏结构及功能的影响。材料与方法:选用40只雄性Wistar大鼠随机分为对照组(饲喂正常饲料)、3个不同PCB浓度的实验组(分别饲喂含PCB10-8mol/L、10-7mol/L、10-6mol/L饲料),共4组。各组按上述不同的饲料连续饲喂3个月后建立慢性PCB毒性动物模型,运用光镜、免疫组化及肝生化检测技术研究PCB对大鼠肝结构及功能的影响。结果:PCB对大鼠肝组织结构的损伤与PCB呈剂量依赖性,PCB高剂量组(10-6mol/L)肝结构受损严重;PCB能诱导肝细胞c-fos表达,10-7mol/L、10-6mol/L组肝细胞c-fos的表达明显高于对照组(P<0.05);同时,PCB能促进肝细胞bcl-2的表达,对照组bcl-2为弱阳性表达,10-7mol/L、10-6mol/L组肝细胞bcl-2的表达明显高于对照组;PCB能致肝组织生化功能改变,使血中URIC、TBLL、DBLL、AST、ALT、TP3、LDH增加,其中PCB10-7mol/L、10-6mol/L剂量组上述肝脏生化指标明显高于对照组(P<0.05)。结论:PCB对大鼠肝脏结构及功能产生明显的损伤作用。
BACKGROUND AND AIM： To study the effects of polychlorinated biphenyl（PCB） on rat liver structure and function. ATERIALS AND METHODS： 40 male Wistar rats were divided into four groups , A group was control group , B group （fed with 10 - s mol/L PCB）, C group（ 10 - 7 mol/L PCB）, D group（ 10 - 6 mol/L PCB） as experimental groups.After three months, all rats were killed . Immunohistochemical and biochemical methods were used to observe the effects of PCB on liver structure and function in rats. RESULTS： The strutural damage of the liver was related to the dose of PCB, with D group showing more severe damage than that of others. PCB could induce c-fos expression in the liver, which was higher in C and D groups compared with other groups （P 〈 0.05） PCB could promote the expression of bcl-2 in liver, with higher expression in C and D groups than that of A and B groups. PCB also could affect biochemical function of liver, such as URIC,TBLL, DBLL, AST, ALT, TP3, LDH to increase in C and D groups compared with A and B groups. CONCLUSION： PCB could lead to liver injuries in the rats.
出处
《癌变·畸变·突变》
CAS
CSCD
2008年第3期224-226,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
甘肃省自然基金资助项目(ZR-97089)
