摘要
目的:研究兰州地区胃癌与微卫星不稳定性(microsatellite instability,MSI)的关系,从基因组不稳定的角度探讨河西地区胃癌高发可能的发病机制.方法:采用苯酚-氯仿法分别提取43例胃癌和21例胃异型增生及相应对照组织DNA.应用银染PCR-SSCP检测技术检测Bat25、Bat26、D2S123、D5S346及D17S250微卫星位点,并对胃癌及癌前病变组织中MSI进行分析.结果:43例胃癌患者中MSI检出28例,检出率为65.1%.其中MSI-H为8例,MSI-L20例,分别为28.6%和71.4%;21例胃异型增生组织中MSI检出6例,检出率为28.6%.其中MSI-H2例占33.3%;MSI-L4例占66.7%.结论:兰州地区胃癌患者中MSI检出率很高,MSI可能是该地区胃癌高发的另一种分子致癌机制,并可以作为胃癌诊断的一个较为敏感的指标.
AIM： To investigate the relationship between microsatellite instability （MSI） and the gastric cancer and its effect on carcinogenesis in Lanzhou province of China. METHODS： Silver staining single strand conformation polymorphis-polymerease chain reaction （PCR-SSCP） was used to screen MSI markers at 5 loci （Bat25, Bat26, D2S123, D5S346 and D17S250） in the DNA which were all extracted from gastric cancer （n = 43） and dysplasia （n = 21） tissues with Phenol-Chloroform method, in the same way as corresponding normal gastrictissues. MSI was analyzed in the gastric cancer and dysplasia. RESULTS： MSI was detected in 28/43 cases of gastric cancer （65.1%） including 8 MSI-H （28.6%）, and 20 MSI-L （71.4%）. Six out of 21 cases were detected in dysplasia （28.6%）, including 2 MSI-H （33.3%） and 4 MSI-L （66.7%）. CONCLUSION： The incidence of MSI in HEXI gastric cancer is very higher, probably contributive to the multistep gastric carcinogenesis. MSI may be a relatively better marker of gastric cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第15期1517-1521,共5页
World Chinese Journal of Digestology
关键词
胃癌
微卫星不稳定性
聚合酶链式反应-单链构象多态性分析
Gastric cancer
Microsatellite instability
Silver staining single strand conformation polymorphis-polymerease chain reaction
