摘要
背景:由于新鲜标本的缺乏,防腐标本用于实验研究增多。目的:观察新鲜与防腐股骨标本之间的生物力学特性的差异。设计、时间及地点:对比观察试验,于2008-08/09在南方医科大学广东省生物力学重点实验室完成。材料:随机选择地区、种族、性别相同的青壮年新鲜和甲醛浸泡6～12个月的防腐股骨头标本各6个,X射线摄片排除骨折、肿瘤、结核及先天畸形。方法:选择静止站立单腿支撑股骨头受力模型,MTS生物力学测试机测试800N载荷下的股骨近段的载荷-应变关系、垂直位移和刚度,施加在模型上的总载荷为800N,以200N分级增量方式加载;试验机速度控制在1.4mm/min进行加载。主要观察指标:①新鲜与防腐标本的载荷-应变关系。②股骨头的载荷-位移关系。③股骨头的轴向刚度。结果:新鲜和防腐标本在正常生理载荷下的载荷-应变关系都基本呈线性变化;在载荷为800N时,防腐标本的位移明显大于新鲜标本的位移(P<0.001);新鲜标本的轴向刚度大于防腐标本(P<0.001)。结论:由于甲醛对防腐股骨标本骨质的影响,平均应变、垂直位移及刚度均与新鲜标本有明显差异,但仍能有效维持近段股骨的正常力学传递,在新鲜标本的获得日益困难的情况下,防腐股骨标本在一定程度上可以替代新鲜标本进行生物力学研究。
BACKGROUND： More embalmed specimens are used in empirical study due to lack of fresh specimens. OBJECTIVE： To investigate the biomechanical difference between fresh and embalmed femur. DESIGN, TIME AND SETTING： The comparative observation was performed at the Guangdong Key Laboratory of Biomechanics, Southern Medical University from August 2008 to September 2008. MATERIALS： Six fresh femoral specimens and six embalmed femoral specimens kept in formatin for 6-12 months were randomly selected. All of specimens were from the adult cadaver with the same race, and sex, and X-ray was used to exclude fracture, tuberculosis, tumor and hereditary malformation in advance. METHODS： One leg in standing position was used in the measurement. The biomechanical test was performed on the MTS type machine to detect the relation of loading and strain, vertical displacement and rigidity of proximal femur under the total loading of 800 N, with 200 N multi-step incremental cycling loading. The speed of testing machine was controlled 1.4 mm/min. MAIN OUTCOME MEASURES： The relation between load and strain; load-displacement; axial direction rigidity of femur head. RESULTS： The load exhibited linear change with strain under physiological loading in fresh and embalmed specimens. The displacement of embalmed specimens was greater than fresh specimens （P 〈 0.001 ）, but the axial rigidity of fresh femur specimens was larger than embalmed femur specimens （P 〈 0.001）. CONCLUSION： The strains, vertical displacement and rigidity of embalmed were significant difference with that of fresh specimens due to the effect of formalin on femur. However, the embalmed femur could maintain normal transfer of load as the proximal femur; therefore, it is feasible to replace fresh femur specimens to a certain extent in biomechanics experiment.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第20期3924-3928,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广东省科技计划项目资助项目(2007B031003013)~~
