摘要
以耧斗菜(Aquilegia vulgaris)为实验材料,研究了不同外植体以及生长调节物质对其愈伤组织和不定芽再生的影响,实验结果表明:叶片作为外植体,培养基为MS+2.0 mg/L NAA+2.0 mg/L6-BA时,较适宜于诱导愈伤组织,诱导率达到92.6%;诱导不定芽的较适宜培养基为MS+0.1 mg/L NAA+1.0 mg/L6-BA。
The effects of different explants and growth regulating substances to the regeneration of callus and adventitious bud of Aquilegia vulgaris were researched. The results showed that when leaf was chosen as the explant and the culture medium was MS ＋ 2. 0 mg/L NAA ＋ 2.0 mg/L 6 - BA, the callus could be easily induced, the inducement rate reached to 92.6% ; MS ＋ 0.1 mg/L NAA ＋ 1.0mg/L 6 - BA was suitable for the inducement of adventitious bud.
出处
《江西农业学报》
CAS
2009年第7期81-82,86,共3页
Acta Agriculturae Jiangxi
基金
北京林业大学大学生科技创新计划(GCS07013)
关键词
耧斗菜
愈伤组织
不定芽
诱导
Aquilegia vulgaris
Callus
Adventitious bud
Inducement
