摘要
Biolog EcoPlateTM微平板是用于研究微生物群落水平多样性的一种主流研究方法。针对目前国内研究者对Biolog实验结果解读不充分和错误层出不穷的情况,提出了一套改进实验结果信息提取的方法。方法包含两个部分,一是基于微生物生理学和生态学意义上的Biolog碳源分类表,是研究者进行解读的参考依据和索引;二是结合实例,阐释如何根据上述分类表、以环境富集原理为指导,利用排序的统计方法进行结果解译,发掘出更多的信息。
Biolog EcoPlateTM is a mainstream approach of studying community-level physiological profiles of microbes. To correct misunderstanding and insufficiency of experimental result explanation, we announced an amendment of information extraction of Biolog EcoPlateTM, which included two parts: (1) carbon substrates assortment sheets based on microbial physiological metabolism and ecological function, providing referrence and index for researchers; (2) illustration of how to use assorment sheet and ordination method to find out more information, guiding by environmental concentration principle.
出处
《微生物学通报》
CAS
CSCD
北大核心
2009年第7期1083-1091,共9页
Microbiology China
基金
国家"十一五"科技支撑计划课题(No.2006BAC10B05)
关键词
BIOLOG
群落水平多样性
碳源
分类
解读
Biolog EcoPlate, Community-level physiological profiles, Carbon substrates, Classification,Explanation