摘要
目的探讨乳腺癌患者血清血管内皮生长因子C(VEGF-C)水平及癌组织中C-erbB-2蛋白表达及其临床意义。方法选取女性乳腺浸润性导管癌患者和乳腺良性病变患者各62例,采用ELISA法检测患者术前和术后1个月血清VEGF-C水平,免疫组织化学法检测乳腺癌组织C-erbB-2蛋白表达,分析VEGF-C与乳腺癌临床病理特征及C-erbB-2蛋白表达的关系。结果乳腺癌患者术前血清VEGF-C水平为(279.65±17.34)pg/ml,乳腺良性病变患者为(167.26±12.15)pg/ml,两者比较差异有统计学意义(P<0.01)。乳腺癌患者术后1个月血清VEGF-C水平为(209.45±15.23)pg/ml,与其术前比较差异有统计学意义(P<0.01)。VEGF-C水平与原发肿瘤分期有关(P<0.05),与患者年龄、肿瘤大小、有无淋巴结转移、绝经情况及ER和PR的表达无关(P>0.05)。C-erbB-2蛋白在乳腺癌中的表达阳性率为54.84%(34/62),明显高于乳腺良性病变的11.29%(7/62),P<0.01。乳腺癌腋窝淋巴结转移患者的C-erbB-2蛋白表达阳性率(69.44%)明显高于腋窝淋巴结无转移患者(34.62%,P<0.05)。血清VEGF-C水平随癌组织中C-erbB-2阳性表达强度增强而增高,两者呈正相关(r=0.813,P<0.05)。结论乳腺疾病患者血清中VEGF-C水平可能成为鉴别乳腺良、恶性病变的辅助指标之一;C-erbB-2与乳腺癌淋巴结转移有关;在乳腺癌的淋巴转移途径中VEGF-C可能与C-erbB-2存在着协同作用。
Objective To investigate clinical significance of serum VEGF-C level and C erbB-2 protein expression in patients with breast cancer. Methods Sixty-two female patients with breast invasive ductal cancer and breast benign lesion were respectively selected. Serum VEGF-C level was detected by enzyme-linked immunosorbent assay （ELISA） before operation and at one month after operation, and C erbB-2 protein expression in tissues of breast cancer was detected by immunohistochemistry. Then, the relationship between serum VEGF-C level and clinicopathologic characteristics and C-erbB 2 protein expressions wereas analyzed. Results The serum VEGF-C level before operation in breast cancer patients[（279.65±17.34） pg/ml]was significantly higher than that in breast benign lesions patients [（167. 26±12.15） pg/ml], P〈0.01. In breast cancer patients, the serum VEGF-C level before operation was higher than that at one month after operation [（209. 454-18.23） pg/ml], P〈0.01. The serum VEGF level was related to tumor stage （P〈0.05） but not to patient age, tumor size, menopause status , lymph node metastasis or not and ER and PR expression （P〉0. 05）. The positive expression rate of C-erbB-2 protein in breast cancer patients （54. 84%, 34/62） was significantly higher than that in breast benign lesion patients （11.29%, 7/62）, P 〈0 . 01. Moreover, the positive expression rate of C-erbB -2 protein in breast cancer patients with axilla lymph node metastasis （69. 44%） was significantly higher than that without axilla lymph node metastases （34.62 % ）, P〈0.05. The serum VEGF level increased with increasing expression intensity of C-erbB-2 protein and there was positive correlation between them （r=0. 813,P〈0.05）. Conclusions The serum VEGF-C level in breast cancer may be conducted as an assisted marker to differential diagnosis of breast tumor. C-erbB-2 is related to lymph node metastasis of breast cancer patients. There is synergistic effect between VEGF-C and C-erbB-2 in the lymph node metastasis way of breast cancer.
出处
《中国普外基础与临床杂志》
CAS
2009年第7期514-517,共4页
Chinese Journal of Bases and Clinics In General Surgery
