摘要
目的探讨转移抑制基因KiSS-1在人胆囊癌转移过程中的作用及临床意义。方法应用组织芯片和免疫组织化学(EnVision)技术检测KiSS-1在59例胆囊癌(其中肝脏侵犯13例,淋巴结转移13例)、7例癌旁和6例正常胆囊组织中的表达。结果胆囊癌组织中KiSS-1的表达阳性率明显低于正常和癌旁组织(P<0.05)。在胆囊癌中,KiSS-1阳性表达与患者性别、年龄、肿瘤大小、组织学类型、分化程度及淋巴结转移均无关,而与胆囊癌的浸润深度、肝脏侵犯及临床分期(Nevin分期)有关(P<0.01)。KiSS-1在Ⅰ+Ⅱ期和Ⅲ+Ⅳ期以及Ⅴ期胆囊癌组织中的表达阳性率分别为92.3%、57.1%和27.8%,呈明显下降趋势(P=0.002)。结论KiSS-1的表达降低与人胆囊癌的发生及其侵袭、转移过程密切相关,可能参与了胆囊癌的发生、侵袭及转移过程的调控。
Objective To investigate the role of KISS-1 gene in the metastatic process of carcinoma of gallbladder and the clinicopathologic significance of KiSS 1 gene expression in carcinoma of gallbladder. Methods Pathological specimens from 59 gallbladder carcinoma tissues （13 hepatic invasion and 13 lymphatic invasion tissues were included）, matched with 7 para-tumor and 6 normal gallbladder tissues, were examined for the expression of KISS-1 gene by tissue microarray technique and immunohistochemistry （EnVision）. Results The positive rate of KISS-1 expression was down-regulated （P〈0.05） in tumor tissues, as compared with normal and para-tumor tissues. In carcinoma of gallbladder, the expression of KISS-1 had no relationship with the gender, age, tumor size, histological grade or differentiation, and metastasis of lymph node, while was associated with the depth of infiltration, invasion of liver and the clinical stages （Nevin）. In Ⅰ ＋ Ⅱ , Ⅲ ＋ Ⅳ and Ⅴ stage, the positive rates of KISS-1 were 92.3% , 57. 1% and 27.8% respectively, with an undeniably clear lowering tendency （P=0. 002）. Conclusion Down-regu lating expression of KISS-1 is closely associated with the processes of genesis, invasion and metastasis in carcinoma of gallbladder, and may participate in regulating these processes.
出处
《中国普外基础与临床杂志》
CAS
2008年第9期678-681,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
昆山市社会发展科技计划项目(编号: KS-0703)~~
关键词
组织芯片
KISS-1基因
胆囊癌
Tissue microarray KISS-1 gene Carcinoma of gallbladder
