摘要
目的:建立提高大样本低概率的细菌阳性检出率,降低检验工作强度,提高工作效率的一种检测程序。方法:PCR结合细菌的培养、分离、生化、血清鉴定。结果:46140份样品,1538份组合样,PCR检测阳性225份,阳性率为14.63%,检出阳性菌75株,阳性检出率为1.63‰,高于前一年同期的0.97‰,差异有高度显著性(P<0.01)。907份样品PCR法与传统法平行检测结果一致,均为检出阳性菌2株,无假阴性。检测耗时折算PCR法(57 d)仅为传统法(220 d)的25.91%,差异有高度显著性(P<0.01)。结论:PCR作为过筛试验,值得在大样本低概率细菌检验项目中推广应用。
Objective： To establish a detecting Salmonella method, and increase positive rate and reduce work toilworn degree. Methods：PCR and germicuhure were used to detect Salmonella. Results：PCR was positive in 14. 63% samples were detected among the 46140 specimens. Positive rate of Salmonella was 1.63%o. It was more than 2003 （P 〈 0. 01 ）. Results： Of PCR and conventional method were the same. Assay was dispend 57 day for PCR and assay was dispend 220 day for conventional method. Conclusion： PCR can be used to detect Salmonella a amass of samples among status of staff in the food trade and public place.
出处
《中国卫生检验杂志》
CAS
2009年第7期1552-1553,1638,共3页
Chinese Journal of Health Laboratory Technology
关键词
PCR
过筛试验
沙门菌
PCR
Filtered test
Trade staff
Salmonella
