摘要
目的：建立液相色谱-三重四极质谱法（LC/MS/MS）测定鱼肉中残留的7种磺胺类药物：磺胺邻二甲氧嘧啶、磺胺间二甲氧嘧啶、磺胺-5-甲氧嘧啶、磺胺二甲氧嘧啶、磺胺喹恶啉、磺胺嘧啶、磺胺甲恶唑。方法：样品经乙腈超声提取,加异丙醇后浓缩至近干,流动相溶解并定容,用正己烷净化后上LC/MS/MS,用多反应监测模式检测,外标法定量。结果：7种磺胺在浓度5-100μg/L范围内线性关系良好,相关系数均达到r〉0.998。检出限0.16μg/kg。结论：本方法简便、快速、定性准确,适合鱼肉中7种磺胺药物残留测定。
Objective：To establish a method for the determination of seven sulphonamides （ Sulfadoxine, Sulphadimethoxine, Sulfameter, Sulfamethazine, Sulfaquinoxaline, Sulfadiazine, Sulfamethoxazole） residues in fish tissue. Methods：The sample was extracted by acetonitrile and detected by Liquid Chromatography - Triple quadrupole mass spectrometer. Results： The correlation coefficients of linear calibration curves were over 0. 998 within the sulphonamides concentration range of 5 - 100 ug/L. The detection limits was 0. 4 ug/L Conclusion：The method can be routinely used for the determination of seven sulphonamides in fish tissue.
出处
《中国卫生检验杂志》
CAS
2009年第7期1494-1495,共2页
Chinese Journal of Health Laboratory Technology
基金
苏州市科协系统软科学课题项目(200724)
关键词
液相色谱-三重四极质谱
鱼肉
磺胺
残留
Liquid chromatography tandem mass spectrometry
Fish tissue
Sulphonamides
Residue analysis
