摘要
〔目的〕在确保检测质量的前提下,探索蛋白印迹(WB)确认实验阴性对照和阴性样本条带重复使用的可能性,以降低使用HIV抗体确认试剂的成本。〔方法〕严格按照《全国艾滋病检测技术规范》(2004年版)的要求和Genelabs公司生产的HIV1+2型(HIVBLOT2.2)抗体免疫印迹试剂盒提供的使用说明进行试验。用新试剂和已使用过的阴性对照和阴性样本的WB条带分别对8份阴性样本、12份卫生部艾滋病参比实验室提供的HIV抗体阳性样本和20份HIV抗体阳性样本进行HIV抗体确认试验,用该试剂盒提供的弱阳性质控样品对试验样本进行5次重复比对实验。〔结果〕使用过的WB条带与未使用过的WB条带进行HIV抗体确认试验,结果是一致的。〔结论〕该方法一旦在全国近200家HIV抗体确认实验室推广使用,假设每个实验室每周进行1次确认实验,每年将可以为国家节约HIV抗体确认试剂费近200万元人民币。
Objective Only by the premise of guarantee of quality, and to cut down the costs of HIV antibody confirmation experiments, explore the possibilities of re-use in negative comparison and negative samples in WB confirmation experiments. Method The experiment was conducted strictly in term of ＂National HIV Testing Technical Specifications＂ （2004） and the manual of HIVI＋2 （HIV BLOT 2.2） antibody immunoblotting reagent boxes （C, enelabs company）. HIV antibody test to confirm in 8 negative samples, 12 positive HIV antibody samples which provided by the Ministry of AIDS Reference Laboratory and 20 positive HIV antibody samples conducted by using new reagents and bands have beent used in negative comparison and negative samples. The weak positive quality sample was provided by HIV1＋2 （HIV BLOT 2.2） antibody immunoblotting reagent boxes （Genelabs company）, 5 repeated comparing experiments were conducted. Result WB used bands and not be used for HIV antibody comfirmed, the results were consistent. Conclusion On the assumption that one time of confirmation experiment once a week in each laboratory, it is possible to save 2 million yuan of HIV antibody confirmation reagents cost to promote the method in nearly 200 laboratorie of HIV antibody confirmation in China.
出处
《中国国境卫生检疫杂志》
CAS
2009年第3期155-157,共3页
Chinese Journal of Frontier Health and Quarantine
