摘要
目的探讨艾滋病病毒(HIV)抗体检测确认实验免疫印迹试验(WB)是否能够有效区别筛查阳性的可疑标本和阴性标本。方法筛查实验采用酶联免疫吸附试验(ELISA),确认实验采用WB法;采用两样本含量较大时均数u检验方法分析阳性-可疑组及阳性-阴性组的S/CO比值,判断两组是否存在显著性差异。结果分析了92份阳性-可疑标本和63份阳性-阴性标本的S/CO比值,两组之间无显著性差异。结论研究和实践都表明,确认实验WB方法不能有效区别筛查阳性的可疑标本和阴性标本。WB方法在确定可疑标本方面存在相当大的不足。
Objective To study whether confirmation test of HIV antibody （Western blot）was capable of effectively differentiating ‘ suspected＇ and ‘ negative＇ specimens that were all ‘ positive＇ in primary screening test. Methods Primary screening test applied ELISA assay; confirmation test applied Western blot assay. We applied statistic analysis（u-test） to judge whether it was significantly different in S/CO ratios between ‘posltiversuspected＇and ‘positive negative＇ specimens. Results There were 92 ‘positive suspected＇specimens and 63 ‘positive-negative＇specimens. h was not significantly different between these two groups.Conclusion Our study and practice indicated that confirmation test of HIV antibody （Western blot）could not effectively differentiate ‘ suspected＇ and‘negative＇ specimens that were all ‘ positive＇ in primary screening test.WB assay had serious limitation in confirming ‘suspected＇ specimens.
出处
《中国艾滋病性病》
CAS
2006年第1期4-6,共3页
Chinese Journal of Aids & STD
关键词
艾滋病病毒
筛查实验
确认实验
S/CO比值
HIV
Primary screening test of HIV antibody
Confirmation test
S/CO ratios
