摘要
【目的】报告一种新的基因芯片质量控制方法。【方法】采用限制性显示技术制备一种两端具有1段特异通用引物互补序列的探针,分别以不同浓度梯度的荧光标记通用引物(Cy3-UP)和随机引物(Cy3-RP)与芯片杂交,比较两者杂交效率。【结果】Cy3-UP的杂交结果显著优于Cy3-RP的杂交结果,在较低浓度就可显示较强的荧光信号。【结论】这种新的基因芯片质量控制方法具有较好的敏感性和特异性,可以用于基因芯片的质量控制。
[Objective] To develop a new method for microarray quality control. [Methods] DNA probes, which contain a specific sequence complementary with universal primers, were prepared by restriction display PCR. The Cy3-1abeled universal primers （Cy3-UP） and Cy3-labeled random primers （Cy3-RP） were hybridized with probes arrayed on the slides at different concentration levels, respectively. Their hybridization efficiency was compared. [ Results] The signals of Cy3-UP were remarkably trose than those of Cy3 - RP, especially at the low concentration level. [Conclusions] The new method has perfect sensitivity and specificity and can be used for microarray quality control.
出处
《武警医学院学报》
CAS
2009年第9期745-747,F0002,共4页
Acta Academiae Medicinae CPAPF
基金
广州市科技计划项目(2007Z3-E0521)
广东省医学科研基金(B2005076)
关键词
基因芯片
质量控制
通用引物
限制性显示
Microarray
Quality control
Universal primer
Restriction display
