摘要
制备出高纯度的探针,用于诊断基因芯片的打印.采用巢式PCR技术,M13作为外侧引物并自行设计内侧引物,扩增克隆在T载体上的基因片段.可制备出成分单一,上下游仅含19bp和20bp的短载体序列的探针,能使打印出的芯片得到较好的杂交效果.该法充分利用巢式PCR的优点,对制备探针的方法进行改进,且简便快速,能得到更高质量的探针,满足打印芯片的要求.
Probes with high purity were amplified by nested PCR in DNA microarray preparations. Nested PCR was applied to amplify the clones in PMD 18T vector with M13 forward and reverse primers as external primers, while a pair of inner primers were designed. Highquality probes could be isolated containing short Tvector sequence. The DNA microarray, which printed with these probes, showed superior hybridization results. The advantages of nested PCR improved the efficiency of preparing probes to make diagnostic microarrays.
出处
《生命科学研究》
CAS
CSCD
2003年第3期271-274,共4页
Life Science Research
基金
广州市重点科技攻关项目(990448022)
