摘要
[目的]研究组织培养条件下红叶臭椿的遗传变异,为进一步遗传育种提供理论依据。[方法]以组培红叶臭椿苗为试材,通过CTAB法提取其基因组DNA,利用RAPD技术对其遗传稳定性进行相关分析。[结果]由同一母株茎尖再生的组培苗遗传物质稳定,在DNA水平上经RAPD鉴定未见明显变异,保持了母株的特性;遗传聚类结果显示,组培苗之间相似系数较高,母株与7个组培单株之间相似系数平均为0.898 8,变异幅度很小,进一步证实同一母株茎尖培养后的组培苗后代具有较高的遗传稳定性。[结论]茎尖再生植株具有较好的遗传稳定性。
[ Objective ] The genetic variation of Ailanthu altissima Swingle cv. Hongye was studied under tissue culture condition, which provided theoretical basis for further genetic breeding. [ Method ] The genomic DNA was extracted by CTAB method and the genetic stability was analyzed by RAPD using Ailanthu altissima Swingle cv. Hongye as material. [ Result ] The seedlings regenerated from shoot tips appeared without variations as compared with their original plant in substance of heredity and had no difference on the level of DNA tested by RAPD analysis. The genetic clustering results indicated that the culture clonal plants were closer to each other. The average similarity coefficient between the maternal with the offsprings was 0.898 8 and the variable amplitude was small. [ Conclusion] The generation from shoot tip culture had higher genetic stability.
出处
《安徽农业科学》
CAS
北大核心
2009年第23期10900-10901,共2页
Journal of Anhui Agricultural Sciences
基金
泰山学院引进人才项目
关键词
组培
红叶臭椿
RAPD
遗传变异
Tissue culture
Ailanthu altissima Swingle cv. Hongye
RAPD
Genetic variation
