摘要
目的：测定冠心丹参片、冠心丹参胶囊、冠心丹参颗粒和冠心丹参滴丸中丹参酮Ⅱa的含量，并进行比较。方法：以甲醇为溶剂，采用超声法提取上述4种冠心丹参制剂中的丹参酮ⅡA；采用高效液相色谱法测定其含量，色谱柱为Agilent Zorbax C8（150mm×4．6mm，5μm），流动相为甲醇-水（72：28），检测波长为270nm。结果：丹参酮ⅡA的检测浓度在2．76～138μg·mL^-1范围内与峰面积积分值呈良好线性关系（r=0．9999）；冠心丹参片、冠心丹参胶囊、冠心丹参颗粒、冠心丹参滴丸的平均回收率分别为99．29％、100．37％、100．18％、98．71％，RSD分别为1。78％、2．10％、1．55％、1．09％（均n=6）。结论：不同冠心丹参制剂中丹参酮Ⅱn含量差别较大，故应尽快建立其质量标准。
OBJECTIVE： To determine and compare the contents of tanshinone ⅡA in difference dosage form of Guanxin danshen preparations（tablets, capsules, granules and dripping pills） . METHODS： The tanshinone ⅡA in different Guanxin danshen preparations was extracted by ultrasonic extraction with methanol as solvent, and the content of tanshinone ⅡA was determined by HPLC on Agilent Zorbax Cs（150 mm × 4.6 mm, 5μm） column with mobile phase consisted of methanol- water （72 ： 28） under a detection wavelength of 270 nm. RESULTS： The linear ranges for tanshinone ⅡA stood at 2.76-138 μg · mL^-1 （r = 0.999 9）.The average recoveries of Guanxin danshen tablets, Guanxin danshen capsules, Guanxin danshen granules and Guanxin danshen dripping pills were 99.29% , 100.37% , 100.18% and 98.71% , respectively with RSD at 1.78% , 2.10%, 1.55%, and 1.09% （all n = 6）, respectively. CONCLUSION：The content of tanshinone ⅡA in differed greatly in different Guanxin danshen preparations; therefore it is urgent to establish the quality standard for Guanxin danshen preparations as soon as possible.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第21期1640-1642,共3页
China Pharmacy
