摘要
目的测定冠心丹参胶囊中丹酚酸B的含量。方法HPLC法,YWG-C18色谱柱(250mm×4.6mm,10μm),流动相:(甲醇-乙腈)∶(甲酸-水)=36.7∶63.3,其中甲醇∶乙腈=3∶1,甲酸∶水=1∶29,流速:1.0mL/min,检测波长为286nm。结果丹酚酸B浓度在0.1～0.9μg范围内线性关系良好(r=0.9998,n=5)。加样回收率为102.60%,RSD=1.19%(n=5)。结论本实验提取丹酚酸B处理方法简单,测定结果稳定可靠,可作为冠心丹参胶囊中丹酚酸B含量测定方法。
Objective To determine the content of salvianolic acid B in Guanxindanshen Capsules. Method In HPLC procedure the determination was carried out on a YWG-G18 column （250 mm×4.6mm,10μm）, the mobile phase was （methanol-acetonitrile）： （formic acid-water） =36.7：63.3. Methanol：acetonitrile=3：1, and formic acid：water=1：29. The flow velocity was 1.0 mL/min and the detection wavelength was 286 nm. Result The result showed that Salvianolic acid B had a good linear relationship when its concentration was between 0.1-0.9 μg （r=-0.9998, n=5）, and the sample recovery of the procedure was 102.60 %, RSD=1.19 % （n=5）. Conclusion The method is easy and reliable, which can be used for quantity control of salvianolic acid B in Guanxindanshen Capsules.
出处
《北京中医药大学学报（中医临床版）》
2007年第2期39-40,共2页
Journal of Beijing University of Traditional Chinese Medicine
